Female odors stimulate CART neurons in the ventral premammillary nucleus of male rats
Female odors stimulate CART neurons in the ventral premammillary nucleus of male rats
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DOI：
10.1016/j.physbeh.2006.03.032
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发表时间：
2006-06-15
影响因子：
2.9
通讯作者：
Elias, Carol F.
中科院分区：
文献类型：
--
作者：
Cavalcante, Judney C.;Bittencourt, Jackson C.;Elias, Carol F.
众所周知，嗅觉信息会影响男性和女性的性行为。被称为信息素的化学感应化合物会激活不同的大脑通路，诱导先天和刻板的行为，以及荷尔蒙的变化。研究表明，女性气味会诱导同种男性的多个脑核中 Fos 表达，包括腹侧前乳头核 (PMV)。尽管调查较少，但之前的研究表明 PMV 在攻击性行为和性行为中发挥着作用。在这项研究中，我们使用 Fos 蛋白表达作为神经元对雌性气味做出反应的标记，这些神经元对性行为缺乏经验的雄性大鼠暴露于肮脏的床上用品中。我们观察到女性气味在整个 PMV 中诱导强烈的 Fos 免疫反应性。这些神经元中的大多数也表达可卡因和安非他明调节转录本 (CART) 免疫反应性。此外，我们使用原位杂交并观察到，在暴露于女性气味后，CART mRNA 仅在腹侧 PMV 中增加。我们的结果表明，女性气味刺激缺乏经验的雄性 PMV 中 CART 的产生。考虑到 PMV CART 神经元也表达瘦素受体，以及它们投射到与生殖相关的区域的事实，我们假设 PMV CART 神经元整合营养和环境（嗅觉）信息，易于调节男性生殖行为。 (c) 2006 Elsevier Inc. 保留所有权利。
Olfactory information is known to influence both male and female sexual behavior. Chemosensory compounds known as pheromones activate distinct brain pathways, inducing innate and stereotyped behaviors, as well as hormonal changes. Studies have shown that female odors induce Fos expression in various brain nuclei of conspecific males, including the ventral premammillary nucleus (PMV). Although poorly investigated, previous studies have suggested that the PMV plays a role in aggressive and sexual behavior. In this study, we used Fos protein expression as a marker for neurons responsive to female odors in sexual inexperienced male rats exposed to soiled bedding. We observed that female odors induced intense Fos immunoreactivity throughout the PMV Most of these neurons also express cocaine- and amphetamine-regulated transcript (CART) immunoreactivity. In addition, we used in situ hybridization and observed that, following exposure to female odors, CART mRNA increased only in the ventral PMV Our results suggest that female odors stimulate CART production in the PMV of inexperienced males. Considering that the PMV CART neurons also express the leptin receptor, as well as the fact that they project to areas related to reproduction, we hypothesize that PMV CART neurons integrate nutritional and environmental (olfactory) information, being apt to modulate male reproductive behavior. (c) 2006 Elsevier Inc. All rights reserved.