Two NAD-independent L-lactate dehydrogenases drive L-lactate utilization in Pseudomonas aeruginosa PAO1
Two NAD-independent L-lactate dehydrogenases drive L-lactate utilization in Pseudomonas aeruginosa PAO1
复制标题
两种不依赖 NAD 的 L-乳酸脱氢酶驱动铜绿假单胞菌 PAO1 中 L-乳酸的利用
DOI：
10.1111/1758-2229.12666
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发表时间：
2018
影响因子：
3.3
通讯作者：
Gao Chao
中科院分区：
文献类型：
--
作者：
Wang Yujiao;Xiao Dan;Liu Qiuyuan;Zhang Yipeng;Hu Chunxia;Sun Jinkai;Yang Chunyu;Xu Ping;Ma Cuiqing;Gao Chao
铜绿假单胞菌常在囊性纤维化（CF）患者的气道中建立慢性感染。l-乳酸是CF痰中最丰富的碳源，l-乳酸的利用可能对P.在CF患者的肺中存活。在本研究中，参与L-乳酸利用的关键酶P. aerosaPAO 1的特征在于使用合成CF痰液培养基（SCFM）。一种高度保守的膜结合NAD非依赖性l-乳酸脱氢酶（l-iLDH）由lldD（PA 4771）编码，一种新的含黄素的膜结合l-iLDH由lldA（PA 2382）编码，这两种酶都被发现有助于P. aerodynosaPAO1.此外，一个lldDandlldAdouble突变体不能在含有l-乳酸盐作为唯一碳源的培养基中生长。本研究阐明了l-乳酸催化剂的机制和重要性，并证明了第一个假单胞菌属。表达二聚乳酸氧化酶
Pseudomonas aeruginosaoften establishes a chronic infection in the airways of patients with cystic fibrosis (CF).l‐Lactate is the most abundant carbon source in the CF sputum, andl‐lactate utilization may be important forP. aeruginosato survive in the lungs of CF patients. In this study, the key enzymes involved inl‐lactate utilization byP. aeruginosaPAO1 were characterized using the synthetic CF sputum medium (SCFM). A highly conserved membrane‐bound NAD‐independentl‐lactate dehydrogenase (l‐iLDH) encoded bylldD(PA4771) and a novel flavin‐containing membrane‐boundl‐iLDH encoded bylldA(PA2382) were both found to contribute tol‐lactate utilization byP. aeruginosaPAO1. In addition, anlldDandlldAdouble mutant was incapable of growing in a medium containingl‐lactate as the sole carbon source. This study clarifies the mechanism and importance ofl‐lactate catabolism, and demonstrates the firstPseudomonasspp. expressing twol‐lactate‐oxidizing enzymes.