Andrographolide Inhibits Human Umbilical Vein Endothelial Cell Invasion and Migration by Regulating MMP-2 and MMP-9 During Angiogenesis
Andrographolide Inhibits Human Umbilical Vein Endothelial Cell Invasion and Migration by Regulating MMP-2 and MMP-9 During Angiogenesis
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DOI：
10.1615/jenvironpatholtoxicoloncol.v30.i1.40
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发表时间：
2011-01-01
影响因子：
2.4
通讯作者：
Kuttan, Girija
中科院分区：
文献类型：
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作者：
Pratheeshkumar, P.;Kuttan, Girija
血管生成，即从预先存在的血管形成新血管，是肿瘤进展和转移所必需的。使用人脐静脉内皮细胞的研究清楚地表明，给予穿心莲内酯显着抑制内皮细胞增殖、迁移、侵袭和管形成。明胶酶谱分析显示穿心莲内酯对基质金属蛋白酶MMP-2和MMP-9的活化具有抑制作用。该研究表明，穿心莲内酯处理可以抑制B16 F-10黑色素瘤细胞中核因子-κ B的p65、p50和c-Rel亚基以及其他转录因子如c-fos、活化的转录因子-2和环磷酸腺苷反应元件结合蛋白的活化和核转位。所有这些结果表明，穿心莲内酯通过抑制MMP-2和MMP-9以及调节转录因子的核转位来抑制体外血管生成。
Angiogenesis, the formation of new blood vessels from preexisting blood vessels, is essential for tumor progression and metastasis. Studies using human umbilical vein endothelial cells clearly demonstrated that administration of andrographolide significantly retarded endothelial cell proliferation, migration, and invasion and tube formation. Gelatin zymographic analysis showed the inhibitory effect of andrographolide on the activation of matrix metalloproteinases MMP-2 and MMP-9. The study reveals that andrographolide treatment could inhibit the activation and nuclear translocation of p65, p50, and c-Rel subunits of nuclear factor-kappa B, and other transcription factors such as c-fos, activated transcription factor-2, and cyclic adenosine monophosphate response element binding protein in B16F-10 melanoma cells. All these results demonstrate that andrographolide inhibits in vitro angiogenesis by inhibiting MMP-2 and MMP-9, and also by regulating the nuclear translocation of transcription factors.