The two RNA polymerases encoded by the nuclear and the plastid compartments transcribe distinct groups of genes in tobacco plastids
The two RNA polymerases encoded by the nuclear and the plastid compartments transcribe distinct groups of genes in tobacco plastids
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DOI：
10.1093/emboj/16.13.4041
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发表时间：
1997-07-01
期刊：
影响因子：
11.4
通讯作者：
Maliga, P
中科院分区：
文献类型：
--
作者：
Hajdukiewicz, PTJ;Allison, LA;Maliga, P
光合高等植物质体基因组编码一种大肠杆菌样RNA聚合酶（PEP）的亚基，该聚合酶起始于大肠杆菌。coli sigma（70）型启动子。我们以前已经证实在光合高等植物中存在第二个核编码的质体RNA聚合酶（NEP）。我们在这里报告许多质体基因和操纵子至少有一个PEP和NEP（Class II转录单位）启动子。然而，质体基因的子集，包括光系统I和II基因，仅从PEP启动子转录（I类基因），而在某些情况下（例如accD）基因仅由NEP转录（III类基因）。通过序列比对发现NEP启动子在转录起始位点附近有一个10个核苷酸的共有序列，本文报道的不同的NEP和PEP启动子通过两种RNA聚合酶的识别提供了组特异性基因表达的一般机制。
The plastid genome in photosynthetic higher plants encodes subunits of an Escherichia coli-like RNA polymerase (PEP) which initiates transcription from E. coli sigma(70)-type promoters. We have previously established the existence of a second nuclear-encoded plastid RNA polymerase (NEP) in photosynthetic higher plants, We report here that many plastid genes and operons have at least one promoter each for PEP and NEP (Class II transcription unit). However, a subset of plastid genes, including photosystem I and II genes, are transcribed from PEP promoters only (Class I genes), while in some instances (e.g. accD) genes are transcribed exclusively by NEP (Class III genes). Sequence alignment identified a 10 nucleotide NEP promoter consensus around the transcription initiation site, Distinct NEP and PEP promoters reported here provide a general mechanism for group-specific gene expression through recognition by the two RNA polymerases.