Large-scale, high-density (up to 512 channels) recording of local circuits in behaving animals
Large-scale, high-density (up to 512 channels) recording of local circuits in behaving animals
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DOI：
10.1152/jn.00785.2013
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发表时间：
2014-03-01
影响因子：
2.5
通讯作者：
Buzsaki, Gyorgy
中科院分区：
文献类型：
--
作者：
Berenyi, Antal;Somogyvari, Zoltan;Buzsaki, Gyorgy
监测行为动物多个脑回路中神经元的代表性部分，对于理解神经元计算是必要的。在这里，我们描述了一种系统，它允许从小体积的神经元组织进行高通道数记录，使用一个允许小型啮齿动物自由行为的轻型信号多路复用头台。该系统集成了多柄、高密度记录硅探头、超灵活互连和微型微驱动器。这些改进允许同时记录数百个地点的局部场电位和单位活动，而不限制动物的自由活动。通过确定海马区和大脑皮层各层和区域的电解剖边界，以及构建真实解剖空间中神经元之间功能联系的电路图，说明了大规模记录的优势。这些方法将允许研究电路操作和依赖行为的区域间相互作用，以测试神经网络和大脑功能的假设。
Monitoring representative fractions of neurons from multiple brain circuits in behaving animals is necessary for understanding neuronal computation. Here, we describe a system that allows high-channel-count recordings from a small volume of neuronal tissue using a lightweight signal multiplexing headstage that permits free behavior of small rodents. The system integrates multishank, high-density recording silicon probes, ultraflexible interconnects, and a miniaturized micro-drive. These improvements allowed for simultaneous recordings of local field potentials and unit activity from hundreds of sites without confining free movements of the animal. The advantages of large-scale recordings are illustrated by determining the electroanatomic boundaries of layers and regions in the hippocampus and neocortex and constructing a circuit diagram of functional connections among neurons in real anatomic space. These methods will allow the investigation of circuit operations and behavior-dependent interregional interactions for testing hypotheses of neural networks and brain function.