Comparison of inflammatory responses in human cells caused by lipopolysaccharides from Escherichia coli and from indigenous bacteria in aquatic environment
Comparison of inflammatory responses in human cells caused by lipopolysaccharides from Escherichia coli and from indigenous bacteria in aquatic environment
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DOI：
10.1080/10934529.2012.695254
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发表时间：
2012-08
期刊：
影响因子：
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通讯作者：
Y. Ohkouchi;S. Tajima;M. Nomura;S. Itoh
中科院分区：
文献类型：
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作者：
Y. Ohkouchi;S. Tajima;M. Nomura;S. Itoh
水样中脂多糖 (LPS) 的内毒素活性通常使用鲎变形细胞裂解物 (LAL) 测定来测定，但众所周知，所测定的活性并不总是代表其在人体中的炎症效力。在这项研究中，使用细胞因子分泌作为生物标志物，比较了用大肠杆菌 LPS、角质形成细胞、CD14+ 单核细胞和 THP-1 刺激的三种不同人类细胞的炎症反应，以开发新的体外测定系统，用于检测水生环境中内毒素的炎症效力的变化。只有刺激 6 小时的 THP-1 在水生环境正常内毒素水平范围内表现出剂量依赖性反应。然后，使用 THP-1 测试了从河水中纯化的环境 LPS 的炎症效力。环境LPS刺激后细胞因子分泌的水平和模式与大肠杆菌LPS完全不同。环境LPS刺激后的白细胞介素8（IL-8）分泌量比大肠杆菌LPS刺激后高约10倍。环境 LPS 还诱导 THP-1 中更高水平的 TNF-α 分泌。这些结果表明，水生环境中脂多糖结构的多样性可能有助于更强且不同的炎症反应。这项研究表明，所提出的 THP-1 测定系统可用于检测水生细菌引起的炎症效力的变化。
The endotoxic activities of lipopolysaccharides (LPSs) in water samples are usually determined using a Limulus amoebocyte lysate (LAL) assay, but it is known that the determined activities do not always represent their inflammatory potency in humans. In this investigation, the inflammatory responses in three different human cells stimulated with Escherichia coli LPS, keratinocyte, CD14+ monocyte, and THP-1, were compared using cytokine secretion as biomarkers to develop novel in vitro assay systems for detecting changes in inflammatory potencies of endotoxins in aquatic environment. Only THP-1 with 6-h stimulation showed dose-dependent responses in the range of normal endotoxin levels in aquatic environment. Then, the inflammatory potency of environmental LPS, which was purified from river water, was tested using THP-1. The levels and patterns of cytokine secretion after the environmental LPS stimulation were completely different from E. coli LPS. Interleukin 8 (IL-8) secretions after the environmental LPS stimulation were approximately 10-fold higher than those after E. coli LPS stimulation. The environmental LPS also induced much higher levels of TNF-α secretions in THP-1. These results suggest that a diversity of LPS structures in aquatic environment could contribute to stronger and different inflammatory responses. This investigation indicated that the proposed THP-1 assay system could be useful for detecting the changes in inflammatory potencies caused by aquatic bacteria.