SEQUENCE ANALYSIS OF THE MURINE HOX-2.2 2.3 AND 2.4 HOMEO BOXES EVOLUTIONARY AND STRUCTURAL COMPARISONS
SEQUENCE ANALYSIS OF THE MURINE HOX-2.2 2.3 AND 2.4 HOMEO BOXES EVOLUTIONARY AND STRUCTURAL COMPARISONS
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DOI：
10.1016/0888-7543(87)90011-5
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发表时间：
1987-01-01
期刊：
影响因子：
4.4
通讯作者：
RUDDLE F H
中科院分区：
文献类型：
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作者：
HART C P;FAINSOD A;RUDDLE F H
我们测定了小鼠11号染色体HOX-2 Homeo box基因复合体(Hox-2.2、-2.3和-2.4)的三个重复排列的鼠框的核苷酸序列和氨基酸序列。分析了与其他测序的同源异型盒的类型和位置的差异。HOX-2.2与其同源人类同源框HU-2几乎完全相同。HOX-2.3与果蝇触角线虫同源结构域和非洲爪哇MM-3同源结构域的61个氨基酸中的59个相同，与人类HuC1的61个氨基酸中的60个相同。Hox-2.3、MM-3和Huc1还共享6个谷氨酸残基，然后是同源基因结构域的15-20个氨基酸3‘终止密码子。HOX-2.4与迄今发现的大多数其他Homeo框相对不同；然而，它在61个位置中的60个位置与Hox-3.1小鼠Homeo结构域匹配。与其他小鼠Homeo结构域的序列比较，以及之前对它们的基因组组织和染色体位置的研究，支持了大规模复制导致两个主要的小鼠Homeo盒基因复合体Hox-1和Hox-2的假说。
We have determined the nucleotide sequences and deduced the amino acid sequences of three tandemly arranged murine boxes of the Hox-2 homeo box gene complex on mouse chromosome 11 (Hox-2.2, -2.3, and -2.4). The type and position of differences with other sequenced homeo boxes were analyzed. Hox-2.2 is nearly identical with its cognate human homeo box Hu-2. Hox-2.3 shares 59 of 61 amino acids with the Antennapedia homeo domain of Drosophila and the MM-3 homeo domain of Xenopus and shows 60 of 61 amino acid identity with human HuC1. Hox-2.3, MM-3, and HuC1 also share a stretch of six glutamic acid residues followed by a stop codon 15-20 amino acids 3' of the homeo domain. Hox-2.4 is relatively divergent from most of the other homeo boxes seuenced to date; however, it matches the Hox-3.1 murine homeo domain at 60 of 61 positions. Sequence comparisons with other murine homeo domains, together with previous studies of their genomic organization and chromosomal location, provide support for the hypothesis of a large-scale duplication resulting in the two major murine homeo box gene complexes Hox-1 and Hox-2.