Cytokine-induced nuclear translocation of signaling proteins and their analysis using the inducible translocation trap system.
Cytokine-induced nuclear translocation of signaling proteins and their analysis using the inducible translocation trap system.
复制标题
细胞因子诱导的信号蛋白核易位及其使用诱导易位陷阱系统的分析。
DOI：
10.1016/j.cyto.2007.11.023
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发表时间：
2008
期刊：
影响因子：
3.8
通讯作者：
Fujii,Hodaka
中科院分区：
文献类型：
--
作者：
SaintFleur,Shella;Fujii,Hodaka
细胞因子与其特异性受体的结合诱导信号转导途径的活化，其中许多涉及信号蛋白的核转位。在这篇综述中，提供了一个概述的姜黄素诱导的信号蛋白的核转位。此外，诱导易位陷阱（ITT），一种新的基于荧光标记的核易位检测系统，及其在核易位蛋白鉴定中的应用进行了阐述。最后，分析“核转位体”，整个一套蛋白质，转位到或出细胞核的细胞外刺激，ITT进行了讨论。
Binding of cytokines to their specific receptors induces activation of signal transduction pathways, many of which involve nuclear translocation of signaling proteins. In this review, an overview of cytokine-induced nuclear translocation of signaling proteins is provided. In addition, inducible translocation trap (ITT), a novel reporter-based system to detect nuclear translocation, and its application for identification of nuclear translocating proteins are elaborated. Finally, analysis of “nuclear translocatome”, the entire set of proteins that translocate into or out of the nucleus in response to extracellular stimuli, by ITT is discussed.