Micro-fractional Epidermal Powder Delivery for Skin Vaccination.
Micro-fractional Epidermal Powder Delivery for Skin Vaccination.
复制标题
用于皮肤疫苗接种的微分表皮粉末输送。
DOI：
10.1007/978-1-4939-3389-1_46
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发表时间：
2016
期刊：
影响因子：
--
通讯作者：
Chen,Xinyuan
中科院分区：
文献类型：
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作者：
Jia,Feng;Liu,Shengwu;Wu,MeiX;Chen,Xinyuan
皮肤富含抗原提呈细胞，是疫苗投放的高度免疫原性部位。在过去的二十年里，几种疫苗已经被批准用于皮肤递送，特别是皮内递送。然而，皮内注射往往会引起频繁和严重的局部反应，阻碍了加入佐剂来进一步促进皮肤接种。在这里，我们描述了一种新的皮肤递送技术，称为微分表皮粉递送或EPD，具有最小的局部反应，以改善皮肤疫苗接种。EPD基于激光或微针治疗，在表皮产生微通道阵列，然后局部应用粉末疫苗涂层阵列贴片，通过微通道将疫苗输送到皮肤。由于分段递送，EPD显著减少了疫苗/佐剂引起的局部反应，而不会影响疫苗的免疫原性和佐剂效力。EPD还消除了针头注射相关的疼痛，并由于直接递送粉末而有望改善疫苗的稳定性。本章描述了在临床前动物模型中进行优势EPD的详细方法。
Skin is a highly immunogenic site for vaccine delivery due to its richness of antigen-presenting cells. Several vaccines have been approved for skin delivery and in particular intradermal delivery in the last two decades. Yet intradermal delivery often causes frequent and severe local reactions, preventing the incorporation of adjuvants to further boost skin vaccination. Here we describe a novel skin delivery technology, called micro-fractional epidermal powder delivery or EPD, with minimized local reactions for improved skin vaccination. EPD is based on laser or microneedle treatment to generate microchannel arrays in the epidermis followed by topical application of powder vaccine-coated array patches to deliver vaccines into the skin via microchannels. Due to the fractional delivery, EPD significantly reduces vaccine/adjuvant-induced local reactions without compromising vaccine immunogenicity and adjuvant potency. EPD also eliminates needle injection-associated pain and is promising to improve vaccine stability due to the direct powder delivery. This chapter describes detailed methods for the advantageous EPD in preclinical animal models.