RELATIVE DISTRIBUTION OF BACTERIA AT CLINICALLY HEALTHY AND PERIODONTALLY DISEASED SITES IN HUMANS
RELATIVE DISTRIBUTION OF BACTERIA AT CLINICALLY HEALTHY AND PERIODONTALLY DISEASED SITES IN HUMANS
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DOI：
10.1111/j.1600-051x.1978.tb01913.x
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发表时间：
1978-01-01
影响因子：
6.7
通讯作者：
HELLDEN, L
中科院分区：
文献类型：
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作者：
LISTGARTEN, MA;HELLDEN, L
从12名患有晚期牙周病的患者中，在每个患者的2个相对正常部位和2个牙周患病部位，用清洁的刮匙采集龈下细菌样本。将样本悬浮在含1%明胶的生理盐水中，并在1小时内通过暗视野显微镜检查。将100 - 200个细菌按百分比分类到以下类别之一：球菌细胞、直杆菌、丝状菌、梭杆菌、弯曲杆菌、小螺旋体、中等大小螺旋体、大螺旋体和能动杆菌。对于每个采样区域，还记录了以下临床标准：牙龈指数、菌斑指数、探诊深度和牙龈液流量。针对每个患者，分别计算正常部位和患病部位的平均值。使用配对t检验比较（双侧α = 0.001），临床正常部位和患病部位的微生物菌群存在显著差异，球菌细胞在正常部位更为优势（74.3%对22.3%），而在患病部位能动杆菌更频繁（12.7%对0.3%），弯曲杆菌（1.7%对0%）、小螺旋体（12.6%对1.1%）、中等大小螺旋体（18.5%对0.5%）和大螺旋体（6.7%对0.2%）也是如此。正常部位能动细胞与非能动细胞的比例为1 : 49，而在患病部位该比例接近1 : 1。在同一患者群体中，健康部位和牙周患病部位相关的菌群不同，这些差异可以通过一种简单且易于应用于临床环境的技术检测出来。以这种方式获得的数据可能有助于监测各种治疗方式对牙周菌群的影响，并可能有助于确定是否存在活动性疾病。
Samples of subgingival bacteria were collected with a clean curette from 2 relatively normal and 2 periodontally diseased sites in each of 12 patients with advanced periodontal disease. The samples were suspended in physiologic saline containing 1% gelatin and examined within 1 h by darkfield microscopy. From 100-200 bacteria were classified on a percentage basis into 1 of the following categories: coccoid cells, straight rods, filaments, fusiforms, curved rods, small spirochetes, medium-sized spirochetes, large spirochetes and motile rods. For each area sampled the following clinical criteria were also recorded: Gingival Index, Plaque Index, probing depth and gingival fluid flow. For each patient separate mean values were calculated for the normal and the diseased sites. Significant differences existed in the microbial flora of clinically normal and diseased sites using a paired t-test comparison (2.alpha. = 0.001), with coccoid cells more predominant at normal sites (74.3% vs. 22.3%), while at diseased sites motile rods were more frequent (12.7% vs. 0.3%), as well as curved rods (1.7% vs. 0%), small spirochetes (12.6% vs. 1.1%), medium-sized spirochetes (18.5% vs. 0.5%) and large spirochetes (6.7% vs. 0.2%). The ratio of motile to non-motile cells in the normal was 1:49, whereas at diseased sites the ratio was in the vicinity of 1:1. A different flora is associated with healthy and periodontally diseased sites in the same patient population and these differences can be detected by means of a technique which is simple and readily adaptable to a clinical setting. The data obtained in this fashion may be useful in monitoring the effect of various treatment modalities on the periodontal flora and possibly in determining the presence or absence of active disease.