The micronucleus assay in human buccal cells as a tool for biomonitoring DNA damage: The HUMN project perspective on current status and knowledge gaps
The micronucleus assay in human buccal cells as a tool for biomonitoring DNA damage: The HUMN project perspective on current status and knowledge gaps
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DOI：
10.1016/j.mrrev.2008.03.007
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发表时间：
2008-07-01
影响因子：
5.3
通讯作者：
Fenech, Michael
中科院分区：
文献类型：
--
作者：
Holland, Nina;Bolognesi, Claudia;Fenech, Michael
脱落口腔细胞的微核 (MN) 测定是监测人类遗传损伤的一种有用且微创的方法。本概述得出的结论是，尽管自 20 世纪 80 年代以来，口腔细胞中的 MN 测定已被用于证明环境和职业暴露、生活方式因素、饮食缺陷和不同疾病的细胞遗传学影响，但关于微核和其他核异常的特征、解释口腔粘膜样本中各种细胞类型的出现以及不同细胞类型的影响的基础生物学仍然存在重要的知识差距。 世界各地实验室的染色程序和评分标准。为了解决这些不确定性，人类微核项目（HUMN；参见 http://www.humn.org）启动了一项新的口腔细胞 MN 检测国际验证项目，类似于之前使用人类淋巴细胞进行的检测。未来的研究应探索检测中变异性的来源（例如实验室和评分者之间，以及受试者个体间和个体内差异），并解决关键技术问题，例如口腔细胞染色方法、正常和变性细胞分类的最佳标准以及微核和其他异常的评分。口腔 MN 测定的统一和标准化将使人群和实验室之间的数据进行更可靠的比较。评估测定的性能。并巩固其在 DNA 损伤生物监测方面的全球应用。 (C) 2008 年由 Elsevier B.V. 出版
The micronucleus (MN) assay in exfoliated buccal cells is a useful and minimally invasive method for monitoring genetic damage in humans. This overview has concluded that although MN assay in buccal cells has been used since the 1980s to demonstrate cytogenetic effects of environmental and occupational exposures, lifestyle factors, dietary deficiencies, and different diseases, important knowledge gaps remain about the characteristics of micronuclei and other nuclear abnormalities, the basic biology explaining the appearance of various cell types in buccal mucosa samples and effects of diverse staining procedures and scoring criteria in laboratories around the world. To address these uncertainties, the human micronucleus project (HUMN; see http://www.humn.org) has initiated a new international validation project for the buccal cell MN assay similar to that previously performed using human lymphocytes. Future research should explore sources of variability in the assay (e.g. between laboratories and scorers, as well as inter- and intra-individual differences in subjects), and resolve key technical issues, such as the method of buccal cell staining, optimal criteria for classification of normal and degenerated cells and for scoring micronuclei and other abnormalities. The harmonization and standardization of the buccal MN assay will allow more reliable comparison of the data among human populations and laboratories. evaluation of the assay's performance. and consolidation of its world-wide use for biomonitoring of DNA damage. (C) 2008 Published by Elsevier B.V.