Fluid exchange across single capillaries in rat intestinal muscle.
Fluid exchange across single capillaries in rat intestinal muscle.
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大鼠肠肌中单个毛细血管的液体交换。
DOI：
10.1152/ajpheart.1982.242.2.h268
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发表时间：
1982
期刊：
影响因子：
--
通讯作者：
Gore,RW
中科院分区：
文献类型：
--
作者：
Gore,RW
先前的研究表明，小肠的肠系膜、肌肉和粘膜区域的液体交换参数存在很大差异。然而，除了肠系膜之外，几乎没有来自不同区域的定量数据可供比较。在本研究中，对大鼠肠肌中的单个毛细血管进行了水力传导率（LP）和闭塞有效压力（P'e）的定量测量。采用Bohlen和Gore的方法通过显微镜观察纵向肠肌的微循环。 Lee、Smaje 和 Zweifach 的单闭塞方法用于估计单毛细血管中的 LP 和 P'e。使用 Lee 分析模型分析实验数据的程序详细信息包含在附录中。在每个研究的毛细管的四个不同位置进行测量，因此可以定量 LP 的梯度并检测 P'e 的变化。毛细血管静脉端的平均 LP 几乎是动脉端 LP 的七倍。动脉末端的平均 LP [距五级小动脉的距离 (%L/Lo) 的 18.6 +/- 2.3(SE)%] 为 1.32 +/- 0.4 X 10(-2) 微米.s-1.cmH2O-1。静脉端的平均 LP (%L/Lo = 81.7 +/- 2.2%) 为 9.15 +/- 0.34 X 10(-2) 微米.s-1.cmH2O-1。中间毛细管位置 39.9 +/- 2.3 和 61.3 +/- 3.2% 处的 LP 值分别为 2.17 +/- 0.34 和 4.48 +/- 0.62 X 10(-2) 微米.s-1.cmH2O-1。所有数据（132 个样品、33 个毛细血管、10 只大鼠）的总平均 LP 为 4.19 +/- 0.61 X 10(-2) 微米.s-1.cmH2O-1。将这些数据与其他组织的结果进行比较表明，肠肌毛细血管的LP是网膜毛细血管的LP的1.7倍，是肠系膜毛细血管的LP的3倍。 P'e 值针对未闭塞状态进行校正，并用于计算总跨毛细血管压力 (delta P)。结果表明，肠道肌肉层水合良好，组织静水压为正值。根据关系式 JVo = LP X delta P 估算毛细血管不同部位的跨毛细血管流体通量 (JVo)。结果表明，当全身动脉压和毛细血管压力正常时，肠肌毛细血管主要是吸收网络。
Previous studies suggest that large differences exist among fluid exchange parameters in the mesenteric, muscle, and mucosal regions of the small intestine. However, few quantitative data from the separate regions, with the exception of the mesentery, are available for comparison. In this study, quantitative measurements of hydraulic conductivities (LP) and occluded effective pressures (P'e) were made on single capillaries in rat intestinal muscle. The microcirculation of longitudinal intestinal muscle was viewed through a microscope using the method of Bohlen and Gore. The single-occlusion method of Lee, Smaje, and Zweifach was used to estimate LP and P'e in single capillaries. Details of the procedures for analyzing the experimental data using the Lee analytical model are included in an APPENDIX. Measurements were made at four different sites in each capillary studied so gradients in LP could be quantitated and variations in P'e could be detected. The average LP at the venous end of the capillaries was nearly seven times greater than LP at the arterial end. The average LP at the arterial end [18.6 +/- 2.3(SE)% of the distance (%L/Lo) from the fifth-order arterioles] was 1.32 +/- 0.4 X 10(-2) micrometers.s-1.cmH2O-1. The average LP at the venous end (%L/Lo = 81.7 +/- 2.2%) was 9.15 +/- 0.34 X 10(-2) micrometers.s-1.cmH2O-1. Values of LP at intermediate capillary locations 39.9 +/- 2.3 and 61.3 +/- 3.2% were 2.17 +/- 0.34 and 4.48 +/- 0.62 X 10(-2) micrometers.s-1.cmH2O-1, respectively. The total mean LP for all the data (132 samples, 33 capillaries, 10 rats) was 4.19 +/- 0.61 X 10(-2) micrometers.s-1.cmH2O-1. Comparison of these data with results from other tissues indicates that LP of intestinal muscle capillaries is 1.7 times greater than LP of omental capillaries and three times greater than LP of mesenteric capillaries. Values of P'e were corrected for the unoccluded state and were used to calculate total transcapillary pressures (delta P). The results suggested that the intestinal muscle layers were well hydrated and that tissue hydrostatic pressures were positive. Transcapillary fluid fluxes (JVo) at different sites on the capillaries were estimated from the relationship, JVo = LP X delta P. The results imply that intestinal muscle capillaries are primarily an absorptive network when systemic arterial pressure and capillary pressures are normal.