Viruses infecting marine picoplancton encode functional potassium ion channels.
Viruses infecting marine picoplancton encode functional potassium ion channels.
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DOI：
10.1016/j.virol.2014.05.002
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发表时间：
2014-10
期刊：
影响因子：
3.7
通讯作者：
F. Siotto;C. Martin;O. Rauh;J. V. Van Etten;I. Schroeder;A. Moroni;G. Thiel
中科院分区：
文献类型：
--
作者：
F. Siotto;C. Martin;O. Rauh;J. V. Van Etten;I. Schroeder;A. Moroni;G. Thiel
藻藻DNA病毒是双链DNA病毒，可感染藻类。它们的大基因组编码许多基因产物，例如小的 K+ 通道，在原核生物和真核生物中具有同源物。对 K+ 通道的筛查显示，它们富含来自淡水栖息地的病毒。最近对来自海洋藻类或南极洲盐水的病毒进行测序，揭示了具有 K+ 通道预测特征的序列，但也有一些意想不到的特征。两个基因编码 78 或 79 个氨基酸蛋白质，这是已知最小的 K+ 通道。同样令人感兴趣的是 K+ 通道中规范 α 螺旋中的不寻常序列。结构预测算法表明新通道具有保守的 α 螺旋折叠，但算法未能识别 K+ 通道孔两侧的预期跨膜域。尽管具有这些意想不到的特性，电生理学研究证实这些新蛋白质是功能性 K+ 通道。
Phycodnaviruses are dsDNA viruses, which infect algae. Their large genomes encode many gene products, like small K+channels, with homologs in prokaryotes and eukaryotes. Screening for K+channels revealed their abundance in viruses from fresh-water habitats. Recent sequencing of viruses from marine algae or from salt water in Antarctica revealed sequences with the predicted characteristics of K+channels but with some unexpected features. Two genes encode either 78 or 79 amino acid proteins, which are the smallest known K+channels. Also of interest is an unusual sequence in the canonical α-helixes in K+channels. Structural prediction algorithms indicate that the new channels have the conserved α-helix folds but the algorithms failed to identify the expected transmembrane domains flanking the K+channel pores. In spite of these unexpected properties electophysiological studies confirmed that the new proteins are functional K+channels.