Behavior, neurochemistry and histology after intranigral lipopolysaccharide injection
Behavior, neurochemistry and histology after intranigral lipopolysaccharide injection
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DOI：
10.1097/00001756-200203040-00006
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发表时间：
2002-03-04
期刊：
影响因子：
1.7
通讯作者：
Hong, JS
中科院分区：
文献类型：
--
作者：
Hsieh, PF;Chia, LG;Hong, JS
帕金森病（PD）患者的黑质（SN）发生炎症和神经元变性。我们研究了黑质内注射脂多糖（LPS）对成年Sprague-Dawley大鼠的影响。第7天进行自发活动测定、神经递质测定和免疫组化灌注固定。双侧LPS注射增加自发活动2至3倍。SN中多巴胺（DA）和5-羟色胺（5-HT）含量降低，二羟苯基乙酸（DOPAC）/DA、高香草酸（HVA）/DA和5-羟吲哚乙酸（5-HIAA）/5-HT比值升高。纹状体DA、DOPAC、HVA、3-甲氧基酪胺和肾上腺素减少，HVA/DA和5-HIAA/SHT比值增加。单侧LPS使同侧多巴胺神经元减少，但对侧增加。本研究首次提供了在PD的LPS模型中行为多动、肾上腺素抑制和神经元可塑性的证据。神经报告13：277-280（C）2002 Lippincott威廉姆斯威尔金斯。
Inflammation and neuronal degeneration of the substantia nigra (SN) occur in Parkinson's disease (PD). We studied the effects of intranigral lipopolysaccharide (LPS) injection on adult Sprague-Dawley rats. Locomotor activity measurement, neurotransmitter determination and perfusion fixation for immunohistochemistry were done on the 7th day. Bilateral LPS injection increased locomotor activity 2- to 3-fold. In the SN, dopamine (DA) and serotonin (5-HT) decreased but the ratios dihydroxyphenyl acetic acid (DOPAC)/DA, homovanillic acid (HVA)/DA and 5-hydroxyindole-acetic acid (5-HIAA)/5-HT increased. In the striatum, DA, DOPAC, HVA, 3-methoxytyramine and epinephrine decreased but HVA/DA and 5-HIAA/SHT ratios increased. Unilateral LPS decreased dopamineric neurons ipsilaterally but increased contralaterally. This study provides the first evidence of behavioral hyperactivity, epinephrine suppression and neuronal plasticity in the LPS model of PD. NeuroReport 13:277-280 (C) 2002 Lippincott Williams Wilkins.