Resolution of ribosomal stalling by ABCF ATPases YfmR and YkpA/YbiT
Resolution of ribosomal stalling by ABCF ATPases YfmR and YkpA/YbiT
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ABCF ATP酶 YfmR 和 YkpA/YbiT 解决核糖体停滞
DOI：
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发表时间：
2024
期刊：
影响因子：
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通讯作者：
V. Hauryliuk
中科院分区：
文献类型：
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作者：
Hiraku Takada;Keigo Fujiwara;G. Atkinson;Chiba Shinobu;V. Hauryliuk
核糖体上蛋白质合成的效率受到组装的氨基酸链的氨基酸组成的强烈影响。测序序列包括富含脯氨酸的基序以及高度带正电荷和负电荷的氨基酸段。长期以来，ABC ATP酶（ABCF）的F亚家族成员一直被假设为促进这些有问题的基序的翻译。在这项研究中，我们应用遗传学和基于PCR的检测来鉴定枯草芽孢杆菌的四种管家ABCF ATP酶：YdiF，YfmM，YfmR/Uup和YkpA/YbiT。我们发现，YfmR合作的翻译因子EF-P，促进翻译的亲丰富的图案。YfmR和EF-P两者的同时损失导致显著的生长缺陷。令人惊讶的是，这种生长缺陷可以在很大程度上被抑制，虽然过表达的EF-P变体缺乏否则至关重要的5-氨基-戊酰化残基K32。使用体内报告分析，我们表明，YfmR的过表达可以减轻核糖体失速Asp-Pro基序。最后，我们证明，YkpA/YbiT促进翻译的正电荷和负电荷的图案，但在解决富含脯氨酸的延伸核糖体失速是无效的。总的来说，我们的研究结果提供了对ABCF翻译因子在调节B蛋白质合成中的功能的深入了解。枯草芽孢杆菌
Efficiency of protein synthesis on the ribosome is strongly affected by the amino acid composition of the assembled amino acid chain. Challenging sequences include proline-rich motifs as well as highly positively and negatively charged amino acid stretches. Members of the F subfamily of ABC ATPases (ABCFs) have been long hypothesised to promote translation of such problematic motifs. In this study we have applied genetics and reporter-based assays to characterise the four housekeeping ABCF ATPases of Bacillus subtilis: YdiF, YfmM, YfmR/Uup and YkpA/YbiT. We show that YfmR cooperates with the translation factor EF-P that promotes translation of Pro-rich motifs. Simultaneous loss of both YfmR and EF-P results in a dramatic growth defect. Surprisingly, this growth defect can be largely suppressed though overexpression of an EF-P variant lacking the otherwise crucial 5-amino-pentanolylated residue K32. Using in vivo reporter assays, we show that overexpression of YfmR can alleviate ribosomal stalling on Asp-Pro motifs. Finally, we demonstrate that YkpA/YbiT promotes translation of positively and negatively charged motifs but is inactive in resolving ribosomal stalls on proline-rich stretches. Collectively, our results provide insights into the function of ABCF translation factors in modulating protein synthesis in B. subtilis.
影响因子：
8.8
作者：
Bruno Pinheiro;C. Scheidler;P. Kielkowski;M. Schmid;I. Forné;Suhui Ye;N. Reiling;E. Takano;A. Imhof;S. Sieber;S. Schneider;K. Jung
通讯作者：
Bruno Pinheiro;C. Scheidler;P. Kielkowski;M. Schmid;I. Forné;Suhui Ye;N. Reiling;E. Takano;A. Imhof;S. Sieber;S. Schneider;K. Jung
影响因子：
16
作者：
Schuller AP;Wu CC;Dever TE;Buskirk AR;Green R
通讯作者：
Green R
影响因子：
5.6
作者：
Lu, Jianli;Deutsch, Carol
通讯作者：
Deutsch, Carol