Bariatric Surgery Alters microRNA Content of Circulating Exosomes in Patients with Obesity
Bariatric Surgery Alters microRNA Content of Circulating Exosomes in Patients with Obesity
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DOI：
10.1002/oby.22379
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发表时间：
2019-02-01
期刊：
影响因子：
6.9
通讯作者：
Kwon, Soon Hyo
中科院分区：
文献类型：
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作者：
Bae, Yun-Ui;Kim, Yongjin;Kwon, Soon Hyo
目的外泌体微小RNA（Exosomal microRNAs，miRNAs）是肥胖的潜在生物标志物，参与肥胖的生物学过程。减肥手术对肥胖患者有健康益处;然而，这些益处的机制尚不清楚。这项研究试图确定与肥胖相关的外泌体miRNA特征以及它在减肥手术后如何变化。方法前瞻性纳入健康志愿者和非糖尿病肥胖患者。该研究使用RNA测序评估了HV和肥胖患者的血清外泌体miRNA谱。为了评估减肥手术的效果，该研究还分析了手术后6个月患者的外泌体miRNA。结果RNA测序结果显示，与HV相比，肥胖患者中有72个外泌体miRNA表达差异，手术前后有41个miRNA表达差异。在差异表达的miRNAs中，该研究确定了9种手术反应miRNAs，与HV相比，这些miRNAs在手术前的患者中高度表达。对9种miRNAs的生物学通路分析表明，它们可能参与WNT、神经营养因子和胰岛素信号传导;胰岛素受体信号级联;以及粘着斑。结论与HV相比，肥胖患者的外泌体miRNA表达谱存在明显差异。此外，手术后体重减轻改变了肥胖患者的外泌体miRNA谱。
Objective Exosomal microRNAs (miRNAs) are potential biomarkers for obesity, in which they regulate biological processes. Bariatric surgery has health benefits for patients with obesity; however, the mechanisms of these benefits are not clear. This study attempted to identify the exosomal miRNA signature associated with obesity and how it changed after bariatric surgery. Methods Healthy volunteers (HVs) and nondiabetic patients with obesity were prospectively enrolled in the study. The study assessed the serum exosomal miRNA profiles of HVs and patients with obesity using RNA sequencing. To evaluate the effects of bariatric surgery, the study also analyzed exosomal miRNAs in patients 6 months after surgery. Results RNA sequencing revealed differential expression of 72 exosomal miRNAs in patients with obesity compared with HVs and differential expression of 41 miRNAs in post- versus presurgery blood. Among the differentially expressed miRNAs, the study identified nine surgery-responsive miRNAs that were highly expressed in patients before surgery compared with HVs. Biological pathway analysis of the nine miRNAs indicated that they are likely involved in WNT, neurotrophin, and insulin signaling; the insulin receptor signaling cascade; and focal adhesion. Conclusions Patients with obesity have a distinct exosomal miRNA expression profile compared with HVs. In addition, weight loss after surgery alters the exosomal miRNA profile of patients with obesity.