The role of nutrient regulation and the Gpa2 protein in the mating pheromone response of C-albicans
The role of nutrient regulation and the Gpa2 protein in the mating pheromone response of C-albicans
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DOI：
10.1111/j.1365-2958.2006.05367.x
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发表时间：
2006-10-01
影响因子：
3.6
通讯作者：
Johnson, Alexander D.
中科院分区：
文献类型：
--
作者：
Bennett, Richard J.;Johnson, Alexander D.
虽然传统上分类为无性，人类真菌病原体白色念珠菌可以进行高效的交配。这种交配的一个关键组成部分是对信息素的反应，这是由一个保守的激酶级联介导的，该激酶级联将信息素受体的信号转导为细胞核中的转录反应。在本文中，我们证明了（i）C.白色念珠菌的α信息素不同的临床分离株，（ii）的反应严重依赖于营养条件，（iii）整个反应是由Ste 2受体介导的，和（iv），在基因诱导方面，响应α信息素在C。白色念珠菌仅显示与酿酒酵母中的响应的边缘重叠。我们进一步研究了信息素诱导的营养控制，并确定GPA 2基因作为一个关键组成部分。我们发现，三角洲gpa 2/三角洲gpa 2突变体是超敏感的信息素，不像野生型菌株，表现出有效的细胞周期停滞（包括形成特征晕固体培养基上）在交配信息素。这些结果表明C. albicans与其他几种真菌相似，但与S.酿酒酵母，整合来自营养感应途径的信号与信息素反应MAP激酶途径的信号，以产生最终的转录反应。
Although traditionally classified as asexual, the human fungal pathogen Candida albicans can undergo highly efficient mating. A key component of this mating is the response to pheromone, which is mediated by a conserved kinase cascade that transduces the signal from the pheromone receptor to a transcriptional response in the nucleus. In this paper we show (i) that the detailed response of C. albicans to the alpha pheromone differs among clinical isolates, (ii) that the response depends critically on nutritional conditions, (iii) that the entire response is mediated by the Ste2 receptor, and (iv) that, in terms of genes induced, the response to alpha pheromone in C. albicans shows only marginal overlap with the response in Saccharomyces cerevisiae. We further investigated the nutritional control of pheromone induction and identify the GPA2 gene as a critical component. We found that Delta gpa2/Delta gpa2 mutants are hypersensitive to pheromone and, unlike wild-type strains, show efficient cell cycle arrest (including the formation of characteristic halos on solid medium) in response to mating pheromone. These results indicate that C. albicans, like several other fungal species but unlike S. cerevisiae, integrates signals from a nutrient-sensing pathway with those of the pheromone response MAP kinase pathway to generate the final transcriptional response.