5-Aminolevolinic acid mitigates the cadmium-induced changes in Brassica napus as revealed by the biochemical and ultra-structural evaluation of roots
5-Aminolevolinic acid mitigates the cadmium-induced changes in Brassica napus as revealed by the biochemical and ultra-structural evaluation of roots
复制标题
根生化和超微结构评估表明，5-氨基左旋叶酸可减轻甘蓝型油菜中镉引起的变化
DOI：
10.1016/j.ecoenv.2013.02.006
复制
发表时间：
2013-06-01
影响因子：
6.8
通讯作者：
Zhou, Weijun
中科院分区：
文献类型：
--
作者：
Ali, Basharat;Tao, Qiaojing;Zhou, Weijun
以油菜为材料，研究了5-氨基乙酰丙酸（ALA）对镉（Cd）胁迫下油菜根系形态和超微结构的改善作用。为了这个目的，植物水培处理在三个不同的镉水平（0，100，500亩M）和叶面喷洒ALA与三个浓度（0，12.5，25毫克/升）同时。结果表明，叶面喷施ALA可改善镉胁迫下的植株生长和根系形态，降低根系活性氧和丙二醛含量。高浓度Cd（500 μ M）处理降低了根中过氧化氢酶（CAT）、超氧化物歧化酶（SOD）、过氧化物酶（POD）和谷胱甘肽还原酶（GR）等抗氧化酶的活性，降低了氧化型谷胱甘肽和总谷胱甘肽的含量。25 mg/l ALA处理显著提高了Cd胁迫下叶片APX、SOD、POD、GSH等抗氧化活性。显微镜下观察表明，外源ALA的应用改善了镉毒害下的细胞结构。在ALA和Cd的联合收割机作用下，细胞核、核膜发达，细胞壁光滑，内质网连续，线粒体形态完整。结果表明，在Cd胁迫下，ALA能显著促进根系生长，提高根系抗氧化酶活性，改善根尖细胞超微结构。(C)2013 Elsevier Inc. All rights reserved.
In the present study, the ameliorating effects of 5-aminolevulinic acid (ALA) under cadmium (Cd) stress conditions were studied with special emphasis on root morphology and ultra-structure in oilseed rape. For this purpose, plants were treated hydroponically at three different Cd levels (0, 100, 500 mu M) and foliar spray of ALA with three concentrations (0, 12.5, 25 mg/l) simultaneously. The results showed that foliar application of ALA improved the plant growth, root morphology and reduced the reactive oxygen species and malondialdehyde contents in roots under Cd stress conditions. The higher concentration of Cd (500 mu M) decreased the activities of antioxidants enzymes like catalase (CAT), superoxide dismutase (SOD), peroxidase (POD) and glutathione reductase (GR) and also reduced the oxidized glutathione and total glutathione contents in roots. Application of ALA at 25 mg/l dosage significantly enhanced the antioxidant activities e.g. APX, SOD, POD, and GSH contents under Cd stress. The microscopic micrographs showed that application of exogenous ALA improved the cell structure under Cd toxicity. A whole cell with developed nucleus, nuclear membrane, smooth cell wall, continuous endoplasmic reticulum, and well shaped mitochondria was observed under the combine application of ALA and Cd. These results suggest that, application of ALA helped the plants to improve root growth, root antioxidant enzymes, and ultra-structural changes in root tip cells under fifteen days Cd-induced stress. (C) 2013 Elsevier Inc. All rights reserved.