Two complementary rat NK cell subsets, Ly49s3+ and NKR-P1B+, differ in phenotypic characteristics and responsiveness to cytokines
Two complementary rat NK cell subsets, Ly49s3+ and NKR-P1B+, differ in phenotypic characteristics and responsiveness to cytokines
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DOI：
10.1189/jlb.0110039
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发表时间：
2010-07-01
影响因子：
5.5
通讯作者：
Inngjerdingen, Marit
中科院分区：
文献类型：
--
作者：
Kveberg, Lise;Jimenez-Royo, Pilar;Inngjerdingen, Marit
大鼠NK细胞的两个主要亚群可以根据其Ly 49 s3或NKRP 1B凝集素样受体的表达来区分。Ly 49 s3（+）NK细胞，而不是NKRP 1B（+）NK细胞，表达广泛的Ly 49受体。在这里，我们研究了这两个子集之间的差异，在他们的某些NK细胞相关分子的表达，以及他们对细胞因子的反应。微阵列分析提示了几个差异表达的基因，包括NKR-P1 B（+）NK细胞优先表达NKG 2A/C受体。这通过用RT.BM1（CD 94/NKG 2的推定配体）的四聚体染色来证实，表明Ly 49和CD 94/NKG 2受体分离成不同的NK细胞区室。此外，与NKRP 1B（+）NK细胞相比，Ly 49 s3（+）NK细胞表达CD 25与响应IL-2的更快增殖相关。因此，某些炎症情况可能优先扩增Ly 49 s3（+）NK细胞。此外，新鲜分离的Ly 49 s3（+）和NKR-P1 B（+）NK细胞产生相似量的细胞因子，并且少量Ly 49 s3（-）NKR-P1 B（-）双阴性NK亚群似乎基于其显著较低的IFN-γ产生而低反应。总的来说，我们的数据证明了NKR-P1 B（+）和Ly 49 s3（+）NK细胞的不同特征，表明了体内不同的任务。J. Leukoc. 88：87-93; 2010.
Two major subsets of rat NK cells can be distinguished based on their expression of the Ly49s3 or the NKRP1B lectin-like receptor. Ly49s3(+) NK cells, but not NKRP1B(+) NK cells, express a wide range of Ly49 receptors. Here, we have examined differences between these two subsets in their expression of certain NK cell-associated molecules as well as their responses to cytokines. A microarray analysis suggested several differentially expressed genes, including preferential expression of NKG2A/C receptors by NKR-P1B(+) NK cells. This was confirmed by staining with tetramers of RT. BM1, the putative ligand of CD94/NKG2, indicating that Ly49 and CD94/NKG2 receptors separate into distinct NK cell compartments. Further, expression of CD25 by Ly49s3(+) NK cells was associated with more rapid proliferation in response to IL-2 as compared with NKRP1B(+) NK cells. Thus, certain inflammatory situations may preferentially expand the Ly49s3(+) NK cells. Moreover, freshly isolated Ly49s3(+) and NKR-P1B(+) NK cells produce similar amounts of cytokines, and a minor Ly49s3(-)NKR-P1B(-) double-negative NK subset appears to be hyporesponsive based on its significantly lower IFN-gamma production. Collectively, our data demonstrate divergent profiles of NKR-P1B(+) and Ly49s3(+) NK cells, indicating distinct tasks in vivo. J. Leukoc. Biol. 88: 87-93; 2010.