Antagonism of nitrous oxide antinociception in mice by intrathecally administered antisera to endogenous opioid peptides
Antagonism of nitrous oxide antinociception in mice by intrathecally administered antisera to endogenous opioid peptides
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DOI：
10.1007/bf02253248
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发表时间：
2000-07-01
影响因子：
11
通讯作者：
Quock, RM
中科院分区：
文献类型：
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作者：
Cahill, FJ;Ellenberger, EA;Quock, RM
此前，在小鼠腹部收缩试验中，一氧化二氮的镇痛作用被证明是由κ阿片受体介导的，由于一氧化二氮被认为会引起内源性阿片肽的神经元释放，从而刺激阿片受体，因此本研究旨在通过确定鞘内（i.t.）施用的抗血清是否可以差异性地抑制一氧化二氮的镇痛作用来鉴定所涉及的阿片肽，特别是在脊髓中。对不同的阿片肽，雄性 NIH Swiss 小鼠进行了 i.t. 预处理。用阿片肽兔抗血清，24小时后暴露于氧气中三种不同浓度的一氧化二氮中的一种，根据数据构建的剂量反应曲线表明，一氧化二氮的镇痛作用被各种强啡肽（DYNs）和甲硫氨酸脑啡肽（ME）的抗血清显着拮抗，但不是β-内啡肽（β-EP）抗血清的拮抗作用。 DYN 和 ME 的抗血清显着提高一氧化二氮镇痛的 AD(50) 值，但不包括 beta-EP。这项研究的这些结果支持这样的假设，即小鼠腹部收缩试验中一氧化二氮的镇痛作用涉及脊髓中 DYN 和 ME 的神经元释放，版权所有 (C) 2000 美国国家科学委员会，ROC 和 S. Karger AG，巴塞尔。
Previously it was demonstrated that nitrous oxide antinociception in the mouse abdominal constriction test is mediated by kappa-opioid receptors, Since nitrous oxide is thought to cause the neuronal release of endogenous opioid peptide to stimulate opioid receptors, this study was designed to identify the opioid peptides involved, especially in the spinal cord, by determining whether nitrous oxide antinociception can be differentially inhibited by intrathecally (i.t.) administered antisera to different opioid peptides, Male NIH Swiss mice were pretreated i.t. with rabbit antisera to opioid peptides then exposed 24 h later to one of three different concentrations of nitrous oxide in oxygen, Dose-response curves constructed from the data indicated that the antinociceptive effect of nitrous oxide was significantly antagonized by antisera to various dynorphins (DYNs) and methionine-enkephalin (ME), but not by antiserum to P-endorphin (beta-EP). The AD(50) values for nitrous oxide antinociception were significantly elevated by antisera to DYNs a nd ME bur not beta-EP, These findings of th is study support the hypothesis that nitrous oxide antinociception in the mouse abdominal constriction test involves the neuronal release of DYN and ME in the spinal cord, Copyright (C) 2000 National Science Council, ROC and S. Karger AG, Basel.