Untargeted Metabolomics Reveals Species-Specific Metabolite Production and Shared Nutrient Consumption by Pseudomonas aeruginosa and Staphylococcus aureus.
Untargeted Metabolomics Reveals Species-Specific Metabolite Production and Shared Nutrient Consumption by Pseudomonas aeruginosa and Staphylococcus aureus.
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DOI：
10.1128/msystems.00480-21
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发表时间：
2021-06-29
期刊：
影响因子：
6.4
通讯作者：
Papin JA
中科院分区：
文献类型：
--
作者：
Dunphy LJ;Grimes KL;Wase N;Kolling GL;Papin JA
虽然已知细菌代谢会影响抗生素的疗效和毒力，但囊性纤维化肺部感染中单个微生物的代谢能力很难从痰液样本中分离出来。在这里，我们表明，非靶向代谢组学分析的多株铜绿假单胞菌和金黄色葡萄球菌的上清液生长在单一培养在合成囊性纤维化培养基（SCFM）揭示了不同的物种特异性的代谢特征，尽管种内代谢变异。我们确定了一组15种代谢产物，这些代谢产物被铜绿假单胞菌和沙门氏菌显著消耗。金黄色葡萄球菌，表明营养竞争有可能影响社区动态，即使在没有其他病原体-病原体相互作用。最后，确定了由一个物种或另一个物种独特产生的代谢物。具体地，在所有测试的铜绿假单胞菌菌株中，均稳健地产生毒力因子前体邻氨基苯甲酸以及喹啉2，4_喹啉二醇（DHQ）。通过对铜绿假单胞菌和S.金黄色葡萄球菌在生理相关的环境中，这项工作提供了对体内代谢相互作用的潜力的见解，并支持开发种属特异性感染诊断标志物。铜绿假单胞菌和S.金黄色葡萄球菌可影响致病性和抗菌功效。在这项研究中，我们的目的是更好地了解铜绿假单胞菌和S之间的代谢相互作用的潜力。金黄色葡萄球菌在类似囊性纤维化肺的环境中。我们发现S.金黄色葡萄球菌和铜绿假单胞菌消耗许多相同的营养物质，这表明代谢竞争可能在共同感染期间的群落动态中起重要作用。我们进一步鉴定了由任一种生物体独特产生的代谢物，这些代谢物有可能发展成为囊性纤维化肺中感染的种属特异性生物标志物。
While bacterial metabolism is known to impact antibiotic efficacy and virulence, the metabolic capacities of individual microbes in cystic fibrosis lung infections are difficult to disentangle from sputum samples. Here, we show that untargeted metabolomic profiling of supernatants of multiple strains of Pseudomonas aeruginosa and Staphylococcus aureus grown in monoculture in synthetic cystic fibrosis media (SCFM) reveals distinct species-specific metabolic signatures despite intraspecies metabolic variability. We identify a set of 15 metabolites that were significantly consumed by both P. aeruginosa and S. aureus, suggesting that nutrient competition has the potential to impact community dynamics even in the absence of other pathogen-pathogen interactions. Finally, metabolites that were uniquely produced by one species or the other were identified. Specifically, the virulence factor precursor anthranilic acid, as well as the quinoline 2,4-quinolinediol (DHQ), were robustly produced across all tested strains of P. aeruginosa. Through the direct comparison of the extracellular metabolism of P. aeruginosa and S. aureus in a physiologically relevant environment, this work provides insight toward the potential for metabolic interactions in vivo and supports the development of species-specific diagnostic markers of infection. IMPORTANCE Interactions between P. aeruginosa and S. aureus can impact pathogenicity and antimicrobial efficacy. In this study, we aim to better understand the potential for metabolic interactions between P. aeruginosa and S. aureus in an environment resembling the cystic fibrosis lung. We find that S. aureus and P. aeruginosa consume many of the same nutrients, suggesting that metabolic competition may play an important role in community dynamics during coinfection. We further identify metabolites uniquely produced by either organism with the potential to be developed into species-specific biomarkers of infection in the cystic fibrosis lung.