Interaction of lymphocytes with lipid bilayer membranes: a model for lymphocyte-mediated lysis of target cells.
Interaction of lymphocytes with lipid bilayer membranes: a model for lymphocyte-mediated lysis of target cells.
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淋巴细胞与脂质双层膜的相互作用：淋巴细胞介导的靶细胞裂解模型。
DOI：
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发表时间：
1975
影响因子：
11.1
通讯作者：
R. Blumenthal
中科院分区：
文献类型：
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作者：
P. Henkart;R. Blumenthal
水平脂质双层膜被用作研究淋巴细胞介导的靶细胞杀伤的模型系统。二硝基苯化的脂质双层可以在物理上支持数十个淋巴细胞超过一小时，而不会破坏或增加膜的电导。然而，在抗Dnp抗体的存在下，人淋巴细胞迅速诱导膜电导增加了几个数量级，而没有膜破裂。这种离子渗透性的增加只发生在淋巴细胞侧的膜voluage为阳性时，就像靶细胞膜的情况一样。这种电导的淋巴细胞和抗体依赖性增加与观察到的抗体包被的靶细胞的淋巴细胞杀伤相似。结果被解释为证据表明，在淋巴细胞杀伤抗体包被的靶细胞的主要事件是在靶膜中的离子传导通道的创建。
Horizontal lipid bilayer membranes were used as a model system to study lymphocyte-mediated killing of target cells. Dinitrophenylated lipid bilayers can physically support dozens of lymphocytes for periods of over one hour without breakage or increasing the electrical conductance of the membrane. However, in the presence of antibody against Dnp, human lymphocytes rapidly induced increases in membrane conductance of several orders of magnitude without membrane breakage. Such ionic permeability increases occurred only when the membrane voluage was positive on the lymphocyte side, as would be the case with a target cell membrane. The lymphocyte and antibody dependence of this conductance increase parallels that observed for lymphocyte killing of antibody-coated target cells. The results are interpreted as evidence that the primary event in lymphocyte killing of antibody-coated target cells is the creation of ion-conducting channels in the target membrane.