A supercritical fluid-based coating technology. 3: Preparation and characterization of bovine serum albumin particles coated with lipids
A supercritical fluid-based coating technology. 3: Preparation and characterization of bovine serum albumin particles coated with lipids
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DOI：
10.1080/02652040210162630
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发表时间：
2003-01-01
影响因子：
3.9
通讯作者：
Benoit, JP
中科院分区：
文献类型：
--
作者：
Dos Santos, IR;Richard, J;Benoit, JP
无溶剂的微粒，加载牛血清白蛋白作为模型蛋白质，使用一种新的超临界（SC）流体为基础的涂层技术。包衣材料由三肉豆蔻酸甘油酯（Dynasan（R）114）或Gelucire（R）50-02组成。对所得微粒的形态、蛋白质含量和体外释放特性进行了表征。由三肉豆蔻酸微针制成的不连续涂层导致30分钟内初始突释率接近70%。然而，在37 ℃的磷酸盐缓冲液中，用Gelucire（R）50-02涂层的颗粒可以在24小时内实现BSA的延长释放。此外，结果表明，在包衣工艺中使用的条件下，SC CO2处理后BSA未发生任何降解。
Solvent-free microparticles, loaded with bovine serum albumin as a model protein, were produced using a novel supercritical (SC) fluid-based coating technology. Coating material consists either of trimyristin (Dynasan(R) 114) or of Gelucire(R) 50-02. Microparticles obtained were characterized as regards their morphology, protein content and in vitro release pro le. A discontinuous coating made of micro-needles of trimyristin led to an initial burst release of similar to70% in 30 min. However, a prolonged release of the BSA could be achieved in a phosphate buffer solution at 37degreesC over a 24 h period from particles coated with Gelucire(R) 50-02. Furthermore, it was shown that BSA does not undergo any degradation after SC CO2 treatment under the conditions used in the coating process.