In vitro selection of yellow passion fruit genotypes for resistance to Fusarium vascular wilt
In vitro selection of yellow passion fruit genotypes for resistance to Fusarium vascular wilt
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DOI：
10.1007/s11240-011-0009-5
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发表时间：
2012-01-01
影响因子：
3
通讯作者：
Bruckner, Claudio Horst
中科院分区：
文献类型：
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作者：
Flores, Patricia Silva;Otoni, Wagner Campos;Bruckner, Claudio Horst
枯萎病（Fusarium oxysporum f.西番莲（Passifloraedulis）是黄果西番莲（Passifloraedulis）栽培的限制因子。由于没有有效的和经济上可行的控制，发展的抗性或至少耐受品种的需求。许多程序已被用于通过真菌培养物滤液或纯化的毒素来初步选择对各种真菌病原体具有抗性的植物基因型。本研究以西番莲种子和试管苗为材料，用不同浓度的尖孢镰刀菌（Fusarium oxysporum f.）西番莲根瘤菌（FOP）培养滤液（0、20、30、40或50%，v/v）或镰刀菌酸（0.10、0.20、0.30或0.40 mM）。随后，用FOP的分生孢子悬浮液接种选定的植物，以评估体内和体外反应之间的相关性。同时比较了对选择剂的体外敏感性和对病原菌的抗性反应。FA、培养滤液和分生孢子悬浮培养处理对根生长有显著影响。观察表明，根是F.尖孢菌属FA和培养滤液处理的抗性基因型的成功离体选择表明，这种策略是可行的，以加速育种西番莲枯萎病抗枯萎病。
Fusarium vascular wilt (caused by Fusarium oxysporum f. sp. passiflorae) is a limiting factor in the cultivation of yellow passion fruit (Passiflora edulis). Since there is no effective and economically viable control available, development of resistant or at least tolerant cultivars are in demand. A number of procedures have been used for the initial selection of plant genotypes resistant to various fungal pathogens by means of a fungal culture filtrate or purified toxin. In this study, seeds and in vitro-grown plantlets of passion fruit were screened with different concentrations of either Fusarium oxysporum f. sp. passiflorae (FOP) culture filtrate (0, 20, 30, 40 or 50%, v/v) or fusaric acid (0.10, 0.20, 0.30 or 0.40 mM) supplemented in Murashige and Skoog (MS) basal media. Subsequently, selected plants were inoculated with a conidial suspension of FOP to assess correlation between in vivo and in vitro responses. In vitro sensitivity to the selective agents and the resistance response to the pathogen were also compared. Root growth was markedly influenced by FA, culture filtrate, and conidial suspension culture treatments. Observations indicated that roots were primary targets for attack by F. oxysporum. Successful in vitro selection of resistant genotypes by both FA and culture filtrate treatments suggested that this strategy was viable for accelerating breeding of passion fruit for resistance to the Fusarium vascular wilt.