BORDETELLA-PERTUSSIS AND BORDETELLA-PARAPERTUSSIS - 2 IMMUNOLOGICALLY DISTINCT SPECIES
BORDETELLA-PERTUSSIS AND BORDETELLA-PARAPERTUSSIS - 2 IMMUNOLOGICALLY DISTINCT SPECIES
复制标题
DOI：
10.1128/iai.61.2.486-490.1993
复制
发表时间：
1993-02-01
影响因子：
3.1
通讯作者：
GUISO, N
中科院分区：
文献类型：
--
作者：
KHELEF, N;DANVE, B;GUISO, N
百日咳杆菌和副百日咳杆菌是近缘关系较近的两个种。两者都是导致人类百日咳暴发的原因，并产生类似的毒力因子，但百日咳毒素是百日咳杆菌特有的。目前的百日咳全细胞疫苗将很快被含有主要粘附素(丝状血凝素和Pertactin)和主要毒素(百日咳毒素)的无细胞疫苗所取代。所有这些因子都是在小鼠呼吸道模型和临床试验中刺激保护性免疫反应的抗原。在本研究中，我们在小鼠呼吸道模型中检测了这些因素以及腺苷环化酶溶血素(另一种百日咳杆菌毒素)对百日咳杆菌感染的保护作用。正如预期的那样，百日咳毒素不能预防副百日咳杆菌的感染，因为这种细菌不表达这种蛋白，但令人惊讶的结果是，其他因素都不能预防副百日咳杆菌的感染。此外，副百日咳杆菌腺苷环化酶溶血素虽然对百日咳杆菌感染有保护作用，但对百日咳杆菌感染没有保护作用。尽管百日咳杆菌和副百日咳杆菌之间高度同源，但没有观察到交叉保护作用。我们的结果概述了这样一个事实，就像在其他革兰氏阴性细菌中一样，波尔德氏菌的表面蛋白在免疫上是不同的。
Bordetella pertussis and Bordetella parapertussis are closely related species. Both are responsible for outbreaks of whooping cough in humans and produce similar virulence factors, with the exception of pertussis toxin, specific to B. pertussis. Current pertussis whole-cell vaccine will soon be replaced by acellular vaccines containing major adhesins (filamentous hemagglutinin and pertactin) and major toxin (pertussis toxin). All of these factors are antigens that stimulate a protective immune response in the murine respiratory model and in clinical assays. In the present study, we examined the protective efficacies of these factors, and that of adenylate cyclase-hemolysin, another B. pertussis toxin, against B. parapertussis infection in a murine respiratory model. As expected, pertussis toxin did not protect against B. parapertussis infection, since this bacterium did not express this protein, but the surprising result was that none of the other factors were protective against B. parapertussis infection. Furthermore, B. parapertussis adenylate cyclase-hemolysin, although it protected against B. parapertussis infection, did not protect against B. pertussis infection. Despite a high degree of homology between both B. pertussis and B. parapertussis species, no cross-protection was observed. Our results outline the fact that, as in other gram-negative bacteria, Bordetella surface proteins vary immunologically.