Distribution of Salmonella in swine herds in Quebec
Distribution of Salmonella in swine herds in Quebec
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DOI：
10.1016/s0378-1135(99)00049-8
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发表时间：
1999-07-01
影响因子：
3.3
通讯作者：
Quessy, S
中科院分区：
文献类型：
--
作者：
Letellier, A;Messier, S;Quessy, S
对之前被确定为受沙门氏菌污染的五个猪精育单位进行了抽样，以确定可能的污染源，并研究沙门氏菌在猪群中的分布。共采集了208个环境样本，其中87个样本(42%)被沙门氏菌污染。从几种类型的样本中发现了沙门氏菌。其中，来自围栏、建筑环境如门、地板、通风装置、灰尘和农场配件的粪便物质最常被发现呈阳性。一些苍蝇和啮齿动物也呈阳性。其中两个收尾单位是综合生产系统和沙门氏菌的流行和分布的一部分。对不同生产工序的一体化设施进行了研究。对41个农场进行了抽样，并对随机选择的围栏中的1923份粪便样本进行了分析。沙门氏菌阳性151例(7.9%)。在抽查的养殖场中，70.7%(29/41)的养殖场沙门氏菌分离阳性。综合生产中不同程度的污染不均匀。母猪换种母猪(15.9%)和母猪育肥单位(21.9%)污染最严重。在生产金字塔中检出10种沙门氏菌血清型(n=132)，以德比沙门氏菌(37.1%)和鼠伤寒沙门氏菌(34.1%)为主。对不同血清型和鼠伤寒沙门氏菌、德比沙门氏菌和阿纳特氏沙门氏菌的不同分离株进行脉冲场凝胶电泳法分析，各血清型内的遗传图谱没有差异，表明在不同的生产步骤中存在垂直污染。(C)1999 Elsevier Science B.V.保留所有权利。
Five porcine finishing units, previously identified as contaminated by Salmonella, were sampled to identify possible sources of contamination and to study the distribution of Salmonella within the herds. A total of 208 environmental samples were taken and 87 samples (42%) were found contaminated by Salmonella spp. Salmonella was recovered from several types of samples. Among these, fecal material from pens, building environment such as doors, floors, ventilation units, dust and farm accessories were most often found positive. Some of the flies and rodents were also positive. Two of the finishing units were part of an integrated production system and the prevalence and distribution of Salmonella spp. at different production steps of the integrated facilities were studied. Forty-one farms were sampled and a total of 1923 faecal samples in randomly selected pens were analysed. One hundred and fifty-one samples (7.9%) were positive for Salmonella spp. Among the farms sampled, 70.7% (29/41) were positive for isolation of Salmonella. The different levels in the integrated production were unevenly contaminated. Replacement sow (15.9%) and finishing unit for gilts (21.9%) were the most contaminated levels. Ten serotypes of Salmonella (n = 132) were identified in the production pyramid with a predominance of Salmonella Derby (37.1%) and Salmonella Typhimurium (34.1%). Pulse Field Gel Electrophoresis analysis of the various isolates from serotypes and Salmonella Typhimurium, Salmonella Derby and Salmonella Anatum showed no variation in the genetic profiles, within each serotype, suggesting a vertical contamination throughout the different production steps. (C) 1999 Elsevier Science B.V. All rights reserved.