Functional characterization of Terminal Flower1 homolog in Cornus canadensis by genetic transformation
Functional characterization of Terminal Flower1 homolog in Cornus canadensis by genetic transformation
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DOI：
10.1007/s00299-019-02369-2
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发表时间：
2019-03-01
影响因子：
6.2
通讯作者：
Xiang, Qiu-Yun (Jenny)
中科院分区：
文献类型：
--
作者：
Liu, Xiang;Zhang, Jian;Xiang, Qiu-Yun (Jenny)
关键信息TFL 1homologeCorcanTFL 1抑制花序发育的起始并调节花序形态在有花植物中，花序形态存在着广泛的变异.尽管生态和进化的重要性，致力于花序形态的遗传基础的进化研究的努力是远远少于那些花的发育。我们以前的基因表达模式的研究提出了一个CorTFL 1-CorAP 1为基础的模式，有限的伞形花序，头，和迷你二歧花从延长花序在山茱萸的进化。本研究通过农杆菌介导法转化加拿大山茱萸，检测了CorcanTFL 1基因在调控花序发育中的作用。我们发现，过表达CorcanTFL 1的转基因植物表现出延迟或抑制花序的起始和发育以及延长的营养生长期。其中CorcanTFL 1已被下调的转基因植物显示出更早的花序出现和苞片和花序大小的减少，叶转化为苞片和腋生叶芽转化为小花序，在最上部的节点轴承花序，或花序分枝和叶从交互对生到互生的叶序变化。这些观察结果支持了CorcanTFL 1在决定开花时间和叶和芽的形态命运的节点轴承花序的重要作用。这些证据与从基因表达模型中预测的CorTFL 1功能一致，支持CorTFL 1在山茱萸花序形式进化分歧中的关键作用。
Key messageTFL1homologCorcanTFL1suppresses the initiation of inflorescence development and regulates the inflorescence morphology inCornus canadensis.AbstractIn flowering plants, there is a wide range of variation of inflorescence morphology. Despite the ecological and evolutionary importance, efforts devoted to the evolutionary study of the genetic basis of inflorescence morphology are far fewer compared to those on flower development. Our previous study on gene expression patterns suggested a CorTFL1-CorAP1 based model for the evolution of determinate umbels, heads, and mini dichasia from elongated inflorescences in Cornus. Here, we tested the function of CorcanTFL1 in regulating inflorescence development in Cornus canadensis through Agrobacterium-mediated transformation. We showed that transgenic plants overexpressing CorcanTFL1 displayed delayed or suppressed inflorescence initiation and development and extended periods of vegetative growth. Transgenic plants within which CorcanTFL1 had been down-regulated displayed earlier emergence of inflorescence and a reduction of bract and inflorescence sizes, conversions of leaves to bracts and axillary leaf buds to small inflorescences at the uppermost node bearing the inflorescence, or phyllotaxy changes of inflorescence branches and leaves from decussate opposite to spirally alternate. These observations support an important role of CorcanTFL1 in determining flowering time and the morphological destinies of leaves and buds at the node bearing the inflorescence. The evidence is in agreement with the predicted function of CorTFL1 from the gene expression model, supporting a key role of CorTFL1 in the evolutionary divergence of inflorescence forms in Cornus.