Chlorhexidine-Loaded Amorphous Calcium Phosphate Nanoparticles for Inhibiting Degradation and Inducing Mineralization of Type I Collagen
Chlorhexidine-Loaded Amorphous Calcium Phosphate Nanoparticles for Inhibiting Degradation and Inducing Mineralization of Type I Collagen
复制标题
用于抑制 I 型胶原蛋白降解和诱导矿化的载有氯己定的无定形磷酸钙纳米颗粒
DOI：
10.1021/acsami.6b14956
复制
发表时间：
2017-04-19
影响因子：
9.5
通讯作者：
Wang, Xiaoyan
中科院分区：
文献类型：
--
作者：
Cai, Xue;Han, Bing;Wang, Xiaoyan
当代牙本质粘合剂的一个主要缺点是其耐用性有限。基质金属蛋白酶（MMP）降解粘接界面内暴露的胶原纤维，导致树脂牙本质粘接老化。在这项研究中，氯己定负载的无定形磷酸钙（ACP）纳米粒子的合成，以诱导胶原纤维的矿化。纳米颗粒可持续释放洗必泰以抑制矿化过程中的MMPs。制备了三种类型的ACP纳米颗粒：不含氯己定的N-ACP、含醋酸氯己定的C-ACP和含葡萄糖酸氯己定的G-ACP，其具有比CACP更高的载药量：扫描和透射电子显微镜表明合成的纳米颗粒具有小于100 nm的直径。有些直径小于40 nm，小于胶原纤维中间隙区的宽度。能量色散X射线光谱，傅立叶变换红外光谱，和高效液相色谱法证实了氯己定的存在下，在nanoparticles。X射线衍射证实纳米颗粒是无定形的。流毒C-ACP的载量为0.11%，G-ACP的载量为0.53%。体外释放曲线表明，氯己定通过一级动力学可持续释放。释放的洗必泰抑制人牙本质粉中胶原的降解，其作用持续时间比相同浓度的纯洗必泰长。ACP可诱导自组装的I型胶原纤维矿化。负载洗必泰的ACP纳米颗粒在适当条件下可持续释放洗必泰和ACP。这对于抑制牙本质胶原原纤维的降解和诱导牙本质胶原原纤维的矿化是有用的。
A major shortcoming of contemporary dentin adhesives is their limited durability. Exposed collagen fibrils Within the bonding interface are degraded by matrix metalloproteinases (MMPs), resulting in aging of the resin dentin bond. In this study, chlorhexidine-loaded amorphous calcium phosphate (ACP) nanoparticies were synthesized to induce the mineralization of collagen fibrils. The nanoparticles sustainably released chlorhexidine to inhibit MMPs during mineralization. Three types of ACP nanoparticies were prepared: N-ACP containing no chlorhexidine, C-ACP containing chlorhexidine acetate, and G-ACP containing chlorhexidine gluconate, whidi had a higher drug-loading than CACP: Scanning and transmission electron microscopy indicated that the synthesized nanoparticles had diameters of less than 100 nm. Some had diameters of less than 40 nm, which was smaller than the width of gap zones in the collagen fibrils. Energy dispersive X-ray spectroscopy, Fourier-transform infrared spectrokopy, and high performance liquid chromatography confirmed the presence of chlorhexidine in the nanoparticies. X-ray diffraction confirmed that the nanoparticles were amorphous. The drug. loading was 0.11% for C-ACP and 0.53% for G-ACP. In vitro release profiles indicated that chlorhexidine was released sustainably via first-order kinetics. Released chlorhexidine inhibited the degradation of collagen in human dentine powder, and its effect lasted longer thari,that of pure chlothexidine of the same concentration. The ACP could induce the mineralization of self assembled type I collagen fibrils. The chlorhexidine-loaded ACP nanoparticies sustainably released chlorhexidine and ACP under appropriate conditions. This is useful for inhibiting degradation and inducing the mineralization of dentine collagen fibrils.