Formation of Ring-Shaped Structures on Erythrocyte Membranes after Treatment with Botulinolysin, a Thiol-Activated Hemolysin from Clostridium botulinum
Formation of Ring-Shaped Structures on Erythrocyte Membranes after Treatment with Botulinolysin, a Thiol-Activated Hemolysin from Clostridium botulinum
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用肉毒杆菌溶血素（一种来自肉毒杆菌的硫醇激活溶血素）处理后红细胞膜上环形结构的形成
DOI：
10.1128/iai.66.6.2987-2990.1998
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发表时间：
1998
影响因子：
3.1
通讯作者：
M. Matsuda
中科院分区：
文献类型：
--
作者：
K. Sekiya;H. Danbara;Y. Futaesaku;Abdul Haque;N. Sugimoto;M. Matsuda
摘要通过电子显微镜研究了肉毒杆菌溶血素（BLY）对红细胞膜的损伤，结果显示环形结构，内径和宽度分别约为32和6.7 nm。BLY在0°C下与膜结合，但随后用戊二醛处理防止在37°C下进一步孵育期间形成环。Zn ~（2+）离子抑制环的形成，但不抑制BLY与膜的结合。
ABSTRACT Damage to erythrocyte membranes by botulinolysin (BLY) was studied by electron microscopy, which revealed ring-shaped structures with inner diameters and widths of approximately 32 and 6.7 nm, respectively. BLY bound to membranes at 0°C, but subsequent treatment with glutaraldehyde prevented ring formation during further incubation at 37°C. Zn2+ ions inhibited ring formation but not binding of BLY to membranes.