Chitosan/polyester-based scaffolds for cartilage tissue engineering: Assessment of extracellular matrix formation
Chitosan/polyester-based scaffolds for cartilage tissue engineering: Assessment of extracellular matrix formation
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DOI：
10.1016/j.actbio.2009.09.006
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发表时间：
2010-03-01
影响因子：
9.7
通讯作者：
Neves, N. M.
中科院分区：
文献类型：
--
作者：
Alves da Silva, M. L.;Crawford, A.;Neves, N. M.
天然来源的聚合物已被广泛用于软骨组织工程支架的生产。本工作的目的是评估和表征细胞外基质（ECM）的形成在两种类型的壳聚糖为基础的支架，使用牛关节软骨细胞（BAC）的影响，这些支架的孔隙率，以及孔径和几何形状，研究了搅拌条件对软骨组织形成的影响，并评估了搅拌条件对ECM形成的影响。使用50%的每种材料的共混物，通过压缩成型和盐浸提来制备（丁二酸丁二醇酯）（CPBS）支架。获得不同孔隙率和孔径结构，使用旋转烧瓶将BAC接种到CPBS支架上。然后将构建体转移到培养箱中，其中一半在搅拌条件下培养。另一半在静态条件下4周通过扫描电子显微镜、组织学程序、I型胶原和II型胶原的免疫定位以及用于糖胺聚糖（GAG）定量的二甲基亚甲基蓝测定来表征构建体。细胞定殖于整个支架并且能够产生ECM具有随机几何形状的大孔改善了蛋白聚糖和II型胶原蛋白的产生然而，该结构对GAG产生具有相反的影响搅拌培养条件表明在两种类型的支架中GAG产生的增强（C）2009 Acta Materialia Inc由Elsevier Ltd.出版。保留所有权利。
Naturally derived polymers have been extensively used in scaffold production for cartilage tissue engineering. The present work aims to evaluate and characterize extracellular matrix (ECM) formation in two types of chitosan-based scaffolds, using bovine articular chondrocytes (BACs) The influence of these scaffolds' porosity, as well as pore size and geometry, on the formation of cartilagineous tissue was studied The effect of stirred conditions on ECM formation was also assessed Chitosan-poly(butylene succinate) (CPBS) scaffolds were produced by compression moulding and salt leaching, using a blend of 50% of each material Different porosities and pore size structures were obtained BACs were seeded onto CPBS scaffolds using spinner flasks. Constructs were then transferred to the incubator, where half were cultured under stirred conditions. and the other half under static conditions for 4 weeks Constructs were characterized by scanning electron microscopy, histology procedures, immunolocalization of collagen type I and collagen type II, and dimethylmethylene blue assay for glycosaminoglycan (GAG) quantification Both materials showed good affinity for cell attachment. Cells colonized the entire scaffolds and were able to produce ECM Large pores with random geometry improved proteoglycans and collagen type II production However, that structure has the opposite effect on GAG production Stirred Culture conditions indicate enhancement of GAG production in both types of scaffold (C) 2009 Acta Materialia Inc Published by Elsevier Ltd. All rights reserved.