An oligonucleotide microchip for genome-wide microRNA profiling in human and mouse tissues
An oligonucleotide microchip for genome-wide microRNA profiling in human and mouse tissues
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DOI：
10.1073/pnas.0403293101
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发表时间：
2004-06-29
影响因子：
11.1
通讯作者：
Croce, CM
中科院分区：
文献类型：
--
作者：
Liu, CG;Calin, GA;Croce, CM
microRNAs（miRNAs）是一类非编码小RNA基因，近年来发现在多种肿瘤中异常表达。在这里，我们描述了一个最近开发的方法学的miRNA基因表达谱的基础上发展的微芯片含有对应于245个miRNA从人类和小鼠基因组的寡核苷酸。我们使用这些微阵列获得了高度可重复的结果，揭示了组织特异性miRNA表达特征，这些数据通过北方印迹、实时RT-PCR和文献检索评估表达而得到证实。微芯片寡核苷酸可以扩展到包括在各种物种中发现的越来越多的miRNA，并且可用于正常和疾病状态的分析。
MicroRNAs (miRNAs) are a class of small noncoding RNA genes recently found to be abnormally expressed in several types of cancer. Here, we describe a recently developed methodology for miRNA gene expression profiling based on the development of a microchip containing oligonucleotides corresponding to 245 miRNAs from human and mouse genomes. We used these microarrays to obtain highly reproducible results that revealed tissue-specific miRNA expression signatures, data that were confirmed by assessment of expression by Northern blots, real-time RT-PCR, and literature search. The microchip oligolibrary can be expanded to include an increasing number of miRNAs discovered in various species and is useful for the analysis of normal and disease states.