Lamina-specific anatomic magnetic resonance imaging of the human retina.
Lamina-specific anatomic magnetic resonance imaging of the human retina.
复制标题
人类视网膜层层特异性解剖磁共振成像。
DOI：
10.1167/iovs.11-7623
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发表时间：
2011
影响因子：
4.4
通讯作者：
Duong,TimothyQ
中科院分区：
文献类型：
--
作者：
Zhang,Yi;Nateras,OscarSanEmeterio;Peng,Qi;Kuranov,RomanV;Harrison,JosephM;Milner,ThomasE;Duong,TimothyQ
目的。人类视网膜的磁共振成像（MRI）面临两大挑战：眼球运动和硬件限制，这可能会阻碍人类视网膜MRI具有足够的时空分辨率。本研究在3-Tesla (T) MRI扫描仪上研究了人眼注视稳定性和高分辨率人体视网膜解剖MRI。方法：采用眼动仪对4名正常志愿者进行MRI注视稳定性评价。在3-T扫描仪上制定了高分辨率MRI （100× 200× 2000 μm）方案。受试者被要求在MRI期间每8秒眨一次眼睛，稳定地盯着一个目标。进行视网膜OCT成像。结果：水平位移和垂直位移的时间SD值分别为78±51 μm和130±51 μm（±SD, n= 4）。MRI在人类视网膜内检测到三层，与啮齿类动物、猫科动物和狒狒视网膜的MRI结果一致。离玻璃体最近的高信号层1可能由神经纤维、神经节细胞和内核层组成；低信号层2、外核层及内外段；高密度的第三层，脉络膜。MRI视网膜/脉络膜厚度为711±37 μm，比OCT厚度（579±34 μm）厚19%（P< 0.05）。本研究报告了人类视网膜层特异性结构的高分辨率MRI。这些初步结果令人鼓舞。时空分辨率有待进一步提高。
Purpose.: Magnetic resonance imaging (MRI) of the human retina faces two major challenges: eye movement and hardware limitation that could preclude human retinal MRI with adequate spatiotemporal resolution. This study investigated eye-fixation stability and high-resolution anatomic MRI of the human retina on a 3-Tesla (T) MRI scanner. Comparison was made with optical coherence tomography (OCT) on the same subjects.Methods.: Eye-fixation stability of protocols used in MRI was evaluated on four normal volunteers using an eye tracker. High-resolution MRI (100× 200× 2000 μm) protocol was developed on a 3-T scanner. Subjects were instructed to maintain stable eye fixation on a target with cued blinks every 8 seconds during MRI. OCT imaging of the retina was performed. Retinal layer thicknesses measured with MRI and OCT were analyzed for matching regions of the same eyes close to the optic nerve head.Results.: The temporal SDs of the horizontal and vertical displacements were 78±51 and 130±51 μm (±SD, n= 4), respectively. MRI detected three layers within the human retina, consistent with MRI findings in rodent, feline, and baboon retinas. The hyperintense layer 1 closest to the vitreous likely consisted of nerve fiber, ganglion cell, and inner nuclear layer; the hypointense layer 2, the outer nuclear layer and the inner and outer segments; and the hyperintense layer 3, the choroid. The MRI retina/choroid thickness was 711±37 μm, 19%(P< 0.05) thicker than OCT thickness (579±34 μm).Conclusions.: This study reports high-resolution MRI of lamina-specific structures in the human retina. These initial results are encouraging. Further improvement in spatiotemporal resolution is warranted.