1H NMR analysis for metabolites in serum and urine from rats administrated chronically with La(NO3)3
1H NMR analysis for metabolites in serum and urine from rats administrated chronically with La(NO3)3
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DOI：
10.1006/abio.2001.5471
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发表时间：
2002-02-01
影响因子：
2.9
通讯作者：
Nie, YX
中科院分区：
文献类型：
--
作者：
Feng, JH;Li, XJ;Nie, YX
H-1 NMR光谱已被用于评估稀土的长期毒理学效应。雄性Wistar大鼠经口给予La（NO3）（3），剂量分别为0.1、0.2、2.0、10和20 mg/kg体重，3-6个月。在1、2和3个月时采集尿液，并在6个月后采集血清样本。在400-和500-MHz H-1 NMR谱上鉴定了大鼠血清和大鼠尿液中的许多低M-r代谢物，包括肌酐、柠檬酸盐、葡萄糖、酮体、三甲胺N-氧化物（TMAO）和各种氨基酸。La 3+诱导的肾脏和肝脏损伤的特征在于排泄的酮体、氨基酸、乳酸盐、乙醇、琥珀酸盐、TMAO、二甲胺和牛磺酸的量增加，以及柠檬酸盐、葡萄糖、尿素和尿囊素的量减少。La（NO3）（3）长期毒性的分子基础信息来自代谢产物排泄的异常模式。血浆中某些生化指标的测定和某些酶的分析支持NMR结果。(C)2002年，Elsevier Science。
H-1 NMR spectroscopy has been used to assess long-term toxicological effects of a rare earth. Male Wistar rats were administrated orally with La(NO3)(3) at doses of 0.1, 0.2, 2.0, 10, and 20 mg/kg body wt, resp., for 3-6 months. Urine was collected at 1, 2, and 3 months and serum samples were taken after 6 months. Numerous low-M-r metabolites in rats serum and rats urine, including creatinine, citrate, glucose, ketone bodies, trimethylamine N-oxide (TMAO), and various amino acids, were identified on 400- and 500-MHz H-1 NMR spectra. La3+-induced renal and liver damage is characterized by an increase in the amounts of the excreted ketone bodies, amino acids, lactate, ethanol, succinate, TMAO, dimethylamine, and taurine and a decrease in citrate, glucose, urea, and allantoin. Information on the molecular basis of the long-term toxicity of La(NO3)(3) was derived from the abnormal patterns of metabolite excretions. An assay of some biochemical indexes and analysis of some enzymes in plasma supported NMR results. (C) 2002 Elsevier Science.