(-)-Deprenyl increases the survival of rat retinal ganglion cells after optic nerve crush.
(-)-Deprenyl increases the survival of rat retinal ganglion cells after optic nerve crush.
复制标题
(-)-Deprenyl 可增加视神经挤压后大鼠视网膜神经节细胞的存活率。
作者：
Y. Buys;G. Trope;W. Tatton
视网膜神经节细胞（retinalganglioncells，RGC）在视神经损伤后可通过凋亡而死亡，而某些神经营养因子可减轻RGC的凋亡。由于（-）-丙炔苯丙胺可以减少一些神经元系统的凋亡死亡，我们确定它是否可以增加视神经挤压后RGC的存活。将荧光逆行示踪剂荧光金（FG）注入成年SD大鼠的两个上级丘，以预先标记视网膜神经节细胞层（RGCL）中的RGC细胞体。四天后，将左侧视神经紧邻地球仪后面压碎，动物每两天通过腹腔注射接受（-）-deprenyl（1 mg/kg）或生理盐水，持续14天。从连续冷冻切片中计数RGCL每mm切片长度的尼氏染色神经元细胞体。通过FG荧光鉴定向上级丘（RGCSCS）发送轴突的RGCL细胞体的数量，作为确定RGCSCS的RGCL神经元（RGCLns）的比例的手段。在未压碎的视网膜中，发现平均40.7%的RGCLn是RGCSCS。用生理盐水处理的未压碎组和用（-）-丙炔苯丙胺处理的未压碎组之间的RGCSCS/mm没有差异。用盐水挤压视神经组显示RGCSCS降低至未挤压盐水组的3.0 +/- 1.0%，而用（-）-丙炔苯丙胺挤压视神经组显示RGCSCS显著降低至未挤压盐水组的36.9 +/- 11.2%。（250字处删节）
Retinal ganglion cells (RGCs) have been shown to die by apoptosis after damage to their axons caused by optic nerve crush and the death can be reduced by some neurotrophic factors. Since (-)-deprenyl can reduce apoptotic death in some neuronal systems, we determined whether it can increase RGC survival after optic nerve crush. Fluoro-Gold (FG), a fluorescent retrograde tracer, was injected into both superior colliculi to pre-label RGC cells bodies in the retinal ganglion cell layers (RGCLs) of adult Sprague-Dawley rats. Fours days later, the left optic nerve was crushed immediately behind the globe and the animals received either (-)-deprenyl (1 mg/kg) or saline by intraperitoneal injection every two days for 14 days. Nissl stained neuronal cell bodies per mm of section length of RGCL were counted from serial frozen sections. The number of RGCL cell bodies sending axons to the superior colliculi (RGCSCS) were identified by FG fluorescence as a means of determining the proportion of RGCL neurons (RGCLns) that were RGCSCS. In the uncrushed retinas an average of 40.7% of the RGCLns were found to be RGCSCS. There was no difference in RGCSCS/mm between the uncrushed treated with saline group and the uncrushed group treated with (-)-deprenyl group. The optic nerve crush with saline group showed a decrease in RGCSCS to 3.0 +/- 1.0% of the uncrushed saline group while the optic nerve crush with (-)-deprenyl group showed a considerably smaller decrease in RGCSCS to 36.9 +/- 11.2% of the uncrushed saline group.(ABSTRACT TRUNCATED AT 250 WORDS)
丙炔苯丙胺对 DSP-4 诱导的神经毒性的保护与其对 MAO B 的抑制无关。
影响因子：
5
作者：
Finnegan,KT;Skratt,JJ;Irwin,I;DeLanney,LE;Langston,JW
通讯作者：
Langston,JW