THE CORTICOTROPIN RELEASING HORMONE GENE IS EXPRESSED IN HUMAN-PLACENTA
THE CORTICOTROPIN RELEASING HORMONE GENE IS EXPRESSED IN HUMAN-PLACENTA
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DOI：
10.1016/s0006-291x(87)80261-9
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发表时间：
1987-11-13
影响因子：
3.1
通讯作者：
MARGIORIS, AN
中科院分区：
文献类型：
--
作者：
GRINO, M;CHROUSOS, GP;MARGIORIS, AN
母体血浆免疫反应性促肾上腺皮质激素释放激素（IR-CRH）随妊娠进行性增加。这种升高的血浆IR-CRH可能是由胎盘分泌的。为了进一步研究这一假设，我们在足月人胎盘中寻找CRH mRNA及其肽产物。使用放射性标记的48聚体寡核苷酸探针互补的一部分人CRH mRNA，我们确定了一个1300个核苷酸的RNA从人胎盘和大鼠下丘脑。我们接下来检查了胎盘IR-CRH的色谱特征。从胎盘中提取的大部分IR-CRH和胎盘片段体外分泌的IR-CRH具有与合成CRH相同的色谱图。这些发现表明CRH基因在人胎盘中表达，并暗示该器官是妊娠期间CRH生物合成的场所。
Maternal plasma immunoreactive corticotropin-releasing hormone (IR-CRH) increases progressively with pregnancy. This elevated plasma IR-CRH is presumably secreted by the placenta. To investigate further this hypothesis, we searched for the CRH mRNA and its peptide product in full term human placentae. Using a radiolabelled 48-mer oligonucleotide probe complementary to a portion of human CRH mRNA, we identified a 1300 nucleotide RNA from human placenta and rat hypothalami. We next examined the chromatographic characteristics of the placental IR-CRH. The bulk of the IR-CRH extracted from placenta and the IR-CRH secreted in vitro by placental fragments had the same chromatographic profiles as synthetic CRH. These findings indicate that the CRH gene is expressed in human placenta and imply that this organ is a site of CRH biosynthesis during pregnancy.