An Optimised Indirect ELISA Protocol for Detection and Quantification of Anti-viral Antibodies in Human Plasma or Serum: A Case Study Using SARS-CoV-2.
An Optimised Indirect ELISA Protocol for Detection and Quantification of Anti-viral Antibodies in Human Plasma or Serum: A Case Study Using SARS-CoV-2.
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DOI：
10.21769/bioprotoc.4905
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发表时间：
2023-12-20
期刊：
影响因子：
0.8
通讯作者：
中科院分区：
文献类型：
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作者：
先进的免疫测定在评估抗体反应、服务于免疫监测目标、描述对不断演变的病毒变异的免疫反应以及指导后续的疫苗接种行动方面至关重要。本方案概述了一种间接ELISA方案，用于检测和定量暴露于病毒抗原后血浆或血清中的病毒特异性抗体。该分析能够测量感兴趣的病毒特异性的IgG、IgA和IgM抗体，提供定性和定量的光密度和浓度数据。虽然该方案涉及SARS-CoV-2，但其方法是通用的，可以修改以评估各种病毒感染的抗体反应并评估疫苗试验结果。•本协议建立在先前描述的专门针对SARS-CoV-2的方法[1]的基础上，并扩大了其对其他病毒感染的适用性。•该方案概述了通过测定血浆或血清的光密度和浓度建立针对SARS-CoV-2感染的抗体反应。
Advanced immunoassays are crucial in assessing antibody responses, serving immune surveillance goals, characterising immunological responses to evolving viral variants, and guiding subsequent vaccination initiatives. This protocol outlines an indirect ELISA protocol to detect and quantify virus-specific antibodies in plasma or serum after exposure to viral antigens. The assay enables the measurement of IgG, IgA, and IgM antibodies specific to the virus of interest, providing qualitative and quantitative optical densities and concentration data. Although this protocol refers to SARS-CoV-2, its methodology is versatile and can be modified to assess antibody responses for various viral infections and to evaluate vaccine trial outcomes. Key features • This protocol builds upon previously described methodology [1] explicitly tailored for SARS-CoV-2 and broadens its applicability to other viral infections. • The protocol outlines establishing antibody responses to SARS-CoV-2 infections by determining optical densities and concentrations from blood plasma or serum.
影响因子：
4.8
作者：
Kitonsa, Jonathan;Kamacooko, Onesmus;Ruzagira, Eugene;Nambaziira, Florence;Abaasa, Andrew;Serwanga, Jennifer;Gombe, Ben;Lunkuse, Jane;Naluyinda, Hadijah;Tukamwesiga, Naboth;Namata, Tamara;Kigozi, Antony;Kafeero, Paddy;Basajja, Vincent;Joseph, Sarah;Pierce, Benjamin F.;Shattock, Robin;Kaleebu, Pontiano
通讯作者：
Kaleebu, Pontiano