A method for parental RNA interference in the wasp Nasonia vitripennis
A method for parental RNA interference in the wasp Nasonia vitripennis
复制标题
DOI：
10.1038/nprot.2006.70
复制
发表时间：
2006-01-01
期刊：
影响因子：
14.8
通讯作者：
Desplan, Claude
中科院分区：
文献类型：
--
作者：
Lynch, Jeremy A.;Desplan, Claude
由于其易于实验室使用，其生物学的独特方面以及其基因组的测序，玻璃鸟黄蜂正在成为一种有用的模式生物，用于解决各种生物学问题。为了充分利用这种生物的潜力，需要操纵基因功能的方法。为此，本文描述了一种玻璃羽蛾亲本RNA干扰（pRNAi）的方案。pRNAi需要向蛹注射双链RNA，允许被注射的黄蜂闭合并检查后代的发育缺陷。这一基本方案是在玻璃翅虫的生命周期背景下描述的。这项技术在阐明迄今为止测试的大多数（尽管不是全部）基因的功能方面很有用，并且在胚胎模式研究之外还有潜在的应用。在Nasonia的pRNAi实验可以在短短2周内完成。
The wasp Nasonia vitripennis is emerging as a useful model organism in which to address a variety of biological questions, due, in part, to its ease of laboratory use, unique aspects of its biology and the sequencing of its genome. In order to take full advantage of the potential of this organism, methods for manipulating gene function are needed. To this end, a protocol for parental RNA interference ( pRNAi) in N. vitripennis is described. pRNAi entails injecting pupae with double-stranded RNA, allowing the injected wasps to eclose and examining the progeny for developmental defects. This basic protocol is described in the context of the life cycle of N. vitripennis. This technique has been useful in elucidating the function of most, although not all, genes tested to date, and has potential applications beyond embryonic patterning. pRNAi experiments in Nasonia can be completed in as little as 2 weeks.