Site-Specific Recognition of Guanosine by Manganese(III) Corroles in DNA Non-Duplex Regions through Active Oxygen Transfer
Site-Specific Recognition of Guanosine by Manganese(III) Corroles in DNA Non-Duplex Regions through Active Oxygen Transfer
复制标题
DNA 非双链体区域中的锰 (III) 咔啉通过活性氧转移对鸟苷进行位点特异性识别
DOI：
10.1002/chem.201101128
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发表时间：
2011-08-01
影响因子：
4.3
通讯作者：
Zhou, Xiang
中科院分区：
文献类型：
--
作者：
Zhai, Qianqian;Xu, Liang;Zhou, Xiang
DNA损伤在细胞过程中起着重要作用。除了天然的蛋白质核酸酶之外，近几十年来，在寻找新的抗癌和抗病毒药物的过程中，已经开发了不同类型的有效的DNA损伤剂。除了双链构型外，DNA结构还包括一些非双链区，这些非双链区被认为是来自DNA复制中的自发错误，因此对细胞起着重要作用。在此，我们专注于这些非双链体区域的DNA和产生的锰（III）的corroles，它表现出高度选择性的切割能力，位于非双链体部分，如环和凸起的鸟苷单元。解理机理是锰诱导的氧化过程。本文给出的结果显示了一种分子方法，可以特异性地探测DNA非双链体结构中的鸟苷单元，因此代表了构建靶向染色体中的非双链体结构的工具的有希望的步骤。
DNA damage plays an important role in cellular processes. Besides natural protein nucleases, different types of efficient agents for DNA damage have been developed over recent decades in the search for new anticancer and antiviral drugs. In addition to the double-stranded configuration, DNA structures also include some non-duplex regions, which are considered to be from spontaneous errors in DNA replication, thus playing an important role for cells. Herein, we focused on these non-duplex regions of DNA and generated manganese(III) corroles, which exhibit a highly selective cleavage ability for guanosine units located at non-duplex portions, such as loops and bulges. The cleavage mechanism was demonstrated to be a manganese-induced oxidation process. The results given herein show a molecular approach that could specifically probe the guanosine units in DNA non-duplex structures, thus representing a promising step in the construction of tools to target non-duplex structures in chromosomes.