Preliminary evidence for the metabolism of benzo(a) pyrene by Plantago lanceolata
Preliminary evidence for the metabolism of benzo(a) pyrene by Plantago lanceolata
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DOI：
10.1023/a:1021240730266
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发表时间：
2003-01-01
影响因子：
4.2
通讯作者：
Lynch, PT
中科院分区：
文献类型：
--
作者：
Brady, CAL;Gill, RA;Lynch, PT
多环芳烃（PAHs）在动物体内转化为活性代谢物可导致DNA损伤，长期以来被认为是潜在的致癌物。在动物体内，多环芳烃的代谢主要发生在肝微粒体中，并与细胞色素p-450介导的混合功能氧化酶（MFO）系统有关。植物中的PAH代谢已被证明是通过类似的酶系统发生的，但相对较少受到关注。本研究旨在探讨植物车前草如何代谢苯并（a）芘（B（a）P），这是一种多环芳烃，其代谢已被广泛研究的动物。这项工作的目的是首先确定B（a）P被吸收，其次确定它被植物生物转化为可能与动物中发现的产物相似的产物。这项工作是通过使用C-14-B（a）P沿着全身放射自显影、闪烁分析和色谱技术来定位B（a）P及其代谢物来实现的。
Polycyclic aromatic hydrocarbons (PAHs) have long been recognised as potential carcinogens in animals in which biotransformation into reactive metabolites can lead to DNA damage. In animals PAHs metabolism mainly occurs in hepatic microsomes and is associated with the cytochrome p-450 mediated mixed functional oxidase (MFO) system. PAH metabolism in plants has been shown to occur via a similar enzyme system, but has received relatively little attention. This study is looking at how the plant species Plantago lanceolata metabolises benzo( a) pyrene (B( a) P), which is one of the PAHs whose metabolism has been studied extensively in animals. The aim of the work is to establish firstly that the B( a) P is taken up and secondly that it is biotransformed by the plant to products possibly similar to those found in animals. This work is achieved by using C-14-B( a) P along with whole body autoradiography, scintillation analysis and chromatography techniques to locate the B(a) P and its metabolites.