The distribution of nitric oxide synthase in the inferior colliculus of guinea pig
The distribution of nitric oxide synthase in the inferior colliculus of guinea pig
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DOI：
10.1016/j.neuroscience.2008.02.030
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发表时间：
2008-06-12
期刊：
影响因子：
3.3
通讯作者：
Rees, A.
中科院分区：
文献类型：
--
作者：
Coote, E. J.;Rees, A.
通过气体一氧化氮调节神经元活动是神经科学中最新的发现之一。在听觉通路中，一氧化氮合酶的最高表达见于下丘（IC），下丘是脑干中行进的平行上行通路和来自听觉皮层的下行投射的会聚的重要中心。在这里，我们使用免疫细胞化学与抗体的神经元型一氧化氮合酶（nNOS），或NOS 1型，映射在IC的豚鼠的nNOS表达的分布。结果表明，nNOS的差异表达的细胞体和神经元跨越其不同的细分。最高水平的神经元染色见于背侧皮质和外侧皮质以及连合核，使神经元的数量增加。它们很容易与中央核的腹外侧部分区分开来，在中央核的腹外侧部分，神经元和胞体中的nNOS表达是最小的。然而，背内侧和尾侧，nNOS表达的区域从背侧皮质延伸到通常指定为中央核的区域，并且nNOS由该细分的特征性神经元表达。我们的研究结果支持细胞性质逐渐转变的想法，而不是中央核和背侧皮质之间的明显边界。这个过渡区可能为这两个亚部之间的功能相互作用提供细胞结构基底。(C)2008年IBRO。由爱思唯尔有限公司出版。保留所有权利。
The modulation of neuronal activity by the gas nitric oxide is one of the most novel discoveries in neuroscience. In the auditory pathway, the highest expression of nitric oxide synthase is found in the inferior colliculus (IC), an important center for the convergence of parallel ascending pathways traveling in the brainstem, and descending projections from the auditory cortex. Here we use immunocytochemistry with an antibody for neuronal nitric oxide synthase (nNOS), or NOS Type 1, to map the distribution of nNOS expression in the IC of the guinea pig. The results show that nNOS is differentially expressed by both cell bodies and neuropil across its different subdivisions. The highest levels of neuronal staining are seen in the dorsal and lateral cortices, and the commissural nucleus, making. them readily distinguishable from the ventro-lateral part of the central nucleus where nNOS expression in neuropil and somata is minimal. Dorso-medially, and caudally, however, the region of nNOS expression extends from the dorsal cortex into the area normally designated as the central nucleus, and nNOS is expressed by neurons characteristic of this subdivision. Our findings support the idea of a gradual transition in cell properties rather than a distinct boundary between the central nucleus and the dorsal cortex. This transition zone may provide a cytoarchitectonic substrate for functional interaction between these two subdivisions. (C) 2008 IBRO. Published by Elsevier Ltd. All rights reserved.