5-Hydroxytryptamine-induced plasma extravasation in the rat knee joint is mediated by multiple prostaglandins.
5-Hydroxytryptamine-induced plasma extravasation in the rat knee joint is mediated by multiple prostaglandins.
复制标题
5-羟色胺诱导的大鼠膝关节血浆外渗是由多种前列腺素介导的。
DOI：
10.1007/s000110300011
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发表时间：
2003
期刊：
影响因子：
--
通讯作者：
Palmer,PPierce
中科院分区：
文献类型：
--
作者：
Xie,G;Wang,Y;Sharma,M;Gabriel,A;Mitchell,J;Xing,Y;Meuser,T;Palmer,PPierce
目的与设计：本研究探讨5-羟色胺（5-HT）诱导的大鼠滑膜血浆外渗是否与前列腺素（PGs）有关。材料与方法：雄性Sprague-Dawley大鼠膝关节灌流5-HT，采用分光光度法测定滑膜毛细血管伊文思蓝染料渗漏量。环氧合酶（考克斯）抑制剂和PG受体亚型选择性拮抗剂的能力，以减少5-HT诱导的滑膜血浆外渗进行了测试。考克斯-1选择性抑制剂SC-560和考克斯-2选择性抑制剂NS-398同样有效，表明两种亚型都参与。选择性的EP 1，EP 2和DP受体亚型的拮抗剂显着减弱5-HT诱导的血浆外渗。然而，选择性的FP，IP和TP亚型的拮抗剂未能减少5-HT诱导的血浆外渗。结论：这些结果表明，多种，但选择性的，PGs亚型介导滑膜血浆外渗产生的5-HT，并建议PGs的下游5-HT的炎症级联反应。
Objective and design:This study investigated whether prostaglandins (PGs) are involved in 5-hydroxytryptamine (5-HT)-induced synovial plasma extravasation.¶Materials and methods:Male Sprague-Dawley rat knee joints were perfused with 5-HT and synovial capillary Evans Blue dye leakage was measured using spectrophotometry. Cyclooxygenase (COX) inhibitors and PG receptor subtype-selective antagonists were tested for the ability to reduce 5-HT-induced synovial plasma extravasation.¶Results:5-HT-induced plasma extravasation was inhibited by indomethacin. The COX-1 selective inhibitor SC-560 and the COX-2 selective inhibitor NS-398 were equally effective, indicating that both isoforms are involved. Antagonists selective for EP1, EP2 and DP receptor subtypes significantly attenuated the 5-HT-induced plasma extravasation. However, antagonists selective for FP, IP and TP subtypes failed to reduce 5-HT-induced plasma extravasation.¶Conclusions:These results demonstrate that multiple, but selective, subtypes of PGs mediate synovial plasma extravasation produced by 5-HT, and suggest that PGs act downstream of 5-HT in the inflammatory cascade.