Rapid detection and surveillance of cfiA-positive Bacteroides fragilis using matrix-assisted laser desorption ionization time-of-flight mass spectrometry
Rapid detection and surveillance of cfiA-positive Bacteroides fragilis using matrix-assisted laser desorption ionization time-of-flight mass spectrometry
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DOI：
10.1016/j.anaerobe.2021.102448
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发表时间：
2021-09-25
期刊：
影响因子：
2.3
通讯作者：
Yanagihara, Katsunori
中科院分区：
文献类型：
--
作者：
Kawamoto, Yasuhide;Kosai, Kosuke;Yanagihara, Katsunori
目的：在 MALDI-TOF MS 系统上使用新的分型软件模块 MALDI Biotyper 分型模块（MBT 分型模块）对 cfiA 阳性脆弱拟杆菌进行监测，并评估该模块的检测能力。方法：使用2006年至2019年分离的脆弱拟杆菌模块推测cfiA阳性菌株。通过PCR确认cfiA基因。在 cfiA 阳性脆弱拟杆菌中，检查了插入序列 (IS) 元件，并进行 MBT STAR-BL 测定以检查美罗培南水解活性。结果：在 396 个脆弱拟杆菌菌株中，MBT 分型模块检测到 33 个假定的 cfiA 阳性菌株 (8.3%)，其中 32 个含有 cfiA 基因。 MBT 分型模块检测 cfiA 阳性脆弱拟杆菌的灵敏度和特异性分别为 100.0% 和 99.7%。在 32 个携带 cfiA 基因的菌株中，7 个菌株拥有 IS 元件，这些元件被认为可以诱导 cfiA 高表达。在 cfiA 和 IS 元件均呈阳性的所有 7 株菌株中均检测到美罗培南水解，并且它们表现出对美罗培南和亚胺培南的耐药性。在 33 株 cfiA 推定阳性菌株中，对美罗培南和亚胺培南的总体不敏感率分别为 84.8% 和 36.4%。结论：MBT 分型模块可以快速准确地检测 cfiA 阳性脆弱拟杆菌，支持其在临床环境中用于监测 cfiA 阳性脆弱拟杆菌。 (c) 2021 Elsevier Ltd. 保留所有权利。
Objectives: To perform surveillance of cfiA-positive Bacteroides fragilis using new subtyping software module, MALDI Biotyper Subtyping Module (MBT Subtyping Module), on MALDI-TOF MS system, and to evaluate the detection ability of the module. Methods: cfiA-positive strains were presumed using the module against B. fragilis isolated between 2006 and 2019. The cfiA gene was confirmed using PCR. In cfiA-positive B. fragilis, the insertion sequence (IS) elements were examined and the MBT STAR-BL assay was performed to examine meropenem hydrolysis activity. Results: Of the 396 B. fragilis strains included, the MBT Subtyping Module detected 33 presumptive cfiA- positive strains (8.3%), of which 32 harbored the cfiA gene. The sensitivity and specificity of the MBT Subtyping Module for detecting cfiA-positive B. fragilis were 100.0% and 99.7%, respectively. Of the 32 strains harboring the cfiA gene, seven strains possessed IS elements, which were thought to induce high cfiA expression. Meropenem hydrolysis was detected in all seven strains that were positive for both cfiA and IS elements, and they exhibited resistance to meropenem and imipenem. The overall non susceptibility rates to meropenem and imipenem were 84.8% and 36.4%, respectively, in the 33 presumptive cfiA-positive strains. Conclusion: The MBT Subtyping Module can detect cfiA-positive B. fragilis rapidly and accurately, supporting its use for surveillance of cfiA-positive B. fragilis in clinical settings. (c) 2021 Elsevier Ltd. All rights reserved.