Neurite growth in PEG gels: Effect of mechanical stiffness and laminin concentration
Neurite growth in PEG gels: Effect of mechanical stiffness and laminin concentration
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DOI：
10.1002/jbm.a.33044
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发表时间：
2011-07-01
影响因子：
4.9
通讯作者：
Willits, Rebecca Kuntz
中科院分区：
文献类型：
--
作者：
Marquardt, Laura;Willits, Rebecca Kuntz
在3D环境中，支架的化学和机械特性可以显着影响神经行为。这些特性如何影响神经细胞对于优化支架内的神经突延伸非常重要。本研究旨在探讨低浓度聚乙二醇（PEG）与层粘连蛋白对离体背根神经节三维生长的影响。由于其对神经突粘附的高亲和力和促进延伸的能力，层粘连蛋白被缀合到PEG链上，并以各种浓度混合在凝胶中以提供化学线索。凝胶刚度，如由G* 测定的，随着PEG浓度的降低和层粘连蛋白缀合物的增加而显著降低。凝胶内的延伸随着层粘连蛋白浓度的增加而增加，层粘连蛋白如何呈递（混合或缀合）到细胞之间没有差异。例如，在3%PEG中，随着层粘连蛋白缀合物浓度从普通增加到100 μ g/ml，延伸从92.29 +/-5.27 μ m增加到146.35 +/-13.12 μ m。结果表明，支架的化学性质影响神经突的生长超过机械性能，因为层粘连蛋白浓度对生长的影响大于凝胶的刚度。神经突长度作为支架刚度和粘附特性的函数也被表征，并证明神经突延伸速率和层粘连蛋白浓度之间存在正线性关系。这项研究进一步证明了表征细胞行为与化学和机械环境之间相互作用的重要性。(C)2011 Wiley Periodicals，Inc. J Biomed Mater Res Part A：98A：1-6，2011.
Within a 3D environment, the chemical and mechanical properties of a scaffold can significantly influence nerve behavior. How these properties influence with nerve cells is important for optimizing neurite extension within a scaffold. The purpose of this study was to investigate the effect of low concentration poly(ethylene glycol) (PEG) with added laminin on 3D growth of dissociated dorsal root ganglia. Because of its high affinity for neurite adhesion and ability to promote extension, laminin was conjugated to the PEG chain, as well as mixed in the gel, at various concentrations to provide chemical cues. Gel stiffness, as determined by G*, significantly decreased with decreasing PEG concentration and with increasing laminin conjugate. Extension within the gels increased as the concentration of laminin increased with no difference between how laminin was presented (mixed or conjugated) to the cells. For example, in 3% PEG, extension increased from 92.29 +/- 5.27 mu m to 146.35 +/- 13.12 lm as laminin conjugate concentration increased from plain to 100 lg/ml. Results indicated that the chemical properties of the scaffold influenced neurite growth more than the mechanical properties as laminin concentration had a greater impact on growth than the stiffness of the gel over the range studied. Neurite length as a function of scaffold stiffness and adhesion properties was also characterized and demonstrated a positive linear relationship between rate of neurite extension and laminin concentration. This study further demonstrates the importance of characterizing interactions between cell behavior and the chemical and mechanical environment. (C) 2011 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 98A: 1-6, 2011.