Turn-on Fluorescence of Davydov-Split Aggregate Particles for Protein Detection
Turn-on Fluorescence of Davydov-Split Aggregate Particles for Protein Detection
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DOI：
10.1016/j.colsurfa.2022.129813
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发表时间：
2022-07
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影响因子：
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通讯作者：
Yi-Fei Ma;Arianna Dicce;N. Reddy;Jiyu Fang
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作者：
Yi-Fei Ma;Arianna Dicce;N. Reddy;Jiyu Fang
有机染料的超分子聚集体通过与特定蛋白质的相互作用来开启荧光，这对于开发生物医学应用的高信噪比荧光探针是非常必要的。本文利用3，3‘-二正十四氧杂碳菁(DiOC14(3))在有/无NaOH的甲醇/水混合溶液中的自组装形成Davydov裂解聚集体粒子。我们发现，在NaOH存在下形成的晶体Davydov裂解聚集体粒子不能对牛血清白蛋白(BSA)做出反应。而在无NaOH条件下形成的非晶态Davydov裂解聚集体粒子则通过与BSA的相互作用逐渐解体。拆解后的DiOC14(3)分子与BSA以单体形式络合，形成扭曲的H-二聚体，从而开启荧光。开启的荧光对BSA具有高度的特异性，并且随着BSA浓度的增加而线性增加。此外，我们还发现，非晶态的Davydov裂解聚集体粒子的荧光行为也可以通过BSA在合成尿液中实现，这突出了它们在检测尿白蛋白方面的潜力。
The turn-on fluorescence of supramolecular aggregates of organic dyes through the interaction with specific proteins is highly desirable for the development of a high signal-to-noise fluorescence probe for biomedical applications. In this paper, we use the self-assembly of 3,3′-ditetradecyloxacarbocyanine (DiOC14(3)) in the methanol/water mixture with/without NaOH to form Davydov-split aggregate particles. We show that the crystalline Davydov-split aggregate particles formed in the presence of NaOH failed to respond to bovine serum albumin (BSA). While the non-crystalline Davydov-split aggregate particles formed in the absence of NaOH gradually disassembled through the interaction with BSA. The disassembled DiOC14(3) molecules complex with BSA in the monomer and twisted H-dimer forms, consequently turning on fluorescence. The turn-on fluorescence is highly specific to BSA and linearly increases with the increase of BSA concentrations. Furthermore, we show that the turn-on fluorescence behavior of non-crystalline Davydov-split aggregate particles by BSA can also be achieved in synthetic urine, which highlights their potential for the detection of urinary albumin.