Endemic syphilis: transfer of resistance to Treponema pallidum strain Bosnia A in hamsters with a cell suspension enriched in thymus-derived cells.
Endemic syphilis: transfer of resistance to Treponema pallidum strain Bosnia A in hamsters with a cell suspension enriched in thymus-derived cells.
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地方性梅毒：用富含胸腺来源细胞的细胞悬液在仓鼠中转移对梅毒螺旋体波斯尼亚 A 株的耐药性。
DOI：
10.1093/infdis/141.6.752
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发表时间：
1980
期刊：
影响因子：
--
通讯作者：
O. Bagasra
中科院分区：
文献类型：
--
作者：
R. Schell;J. Chan;J. Lefrock;O. Bagasra
提供了胸腺衍生（T）细胞参与宿主防御梅毒感染的直接证据。与接受正常淋巴样细胞或仅接受正常骨髓细胞的动物相比，接受来自对梅毒螺旋体菌株波斯尼亚A感染免疫的仓鼠的细胞的经辐照的骨髓重建仓鼠具有显著较低的重量和每个淋巴结较少的密螺旋体。通过玻璃柱和尼龙毛柱连续过滤合并的脾和淋巴结细胞，获得富含免疫仓鼠T细胞的细胞悬液。分级分离的细胞悬浮液对植物血凝素、大肠杆菌脂多糖和硫酸葡聚糖的刺激反应较差，但对T细胞有丝分裂原刀豆球蛋白A的反应相对较强。分离后，抗胸腺细胞血清和补体敏感细胞的比例显着增加。这些去除骨髓来源细胞的免疫细胞悬液在赋予T.苍白球
Direct evidence for involvement of thymus-derived (T) cells in host defense against syphilitic infection is presented. Irradiated, bone marrow-reconstituted hamsters receiving cells from hamsters immune to infection with Treponema pallidum strain Bosnia A had significantly lower weights and fewer treponemes per lymph node than animals that had received normal lymphoid cells or only normal bone marrow cells. Cell suspensions enriched in T cells from immune hamsters were obtained by sequential filtration of pooled spleen and lymph node cells through glass- and nylon-wool columns. The fractionated suspensions of cells responded poorly to stimulation by phytohemagglutinin, Escherichia coli lipopolysaccharide, and dextran sulfate, but responded relatively strongly to the T-cell mitogen, convanavalin A. After fractionation the proportion of cells susceptible to antithymocyte serum and complement increased significantly. These immune cell suspensions depleted of bone marrow-derived cells closely resembled unfractionated suspensions in their ability to confer resistance to challenge with T. pallidium.