Peroxiredoxin-I is an autoimmunogenic tumor antigen in non-small cell lung cancer
Peroxiredoxin-I is an autoimmunogenic tumor antigen in non-small cell lung cancer
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DOI：
10.1016/j.febslet.2005.04.028
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发表时间：
2005-05-23
期刊：
影响因子：
3.5
通讯作者：
Yoo, YJ
中科院分区：
文献类型：
--
作者：
Chang, JW;Lee, SH;Yoo, YJ
在真核细胞中，过氧化还原蛋白既是抗氧化剂，也是 H2O2 介导的信号传导的调节剂。我们之前发现过氧化还原蛋白-I (Prx-I) 在非小细胞肺癌 (NSCLC) 组织中过度表达。由于过度表达的蛋白质可以诱导体液免疫反应，因此我们使用蛋白质印迹检查了 NSCLC 患者的血清是否表现出针对 Prx-I 的免疫反应性。我们发现，53 名接受测试的 NSCLC 患者中有 25 名 (47%) 的血清中含有针对 Prx-I 的自身抗体，而在 50 名健康受试者的 4 名 (8%) 血清中检测到了这种活性。 Prx-I 本身在 53 名 NSCLC 患者中的 18 名 (34%) 血清中检测到，但仅在 50 名对照者的 1 名 (2%) 血清中检测到。此外，17%的NSCLC血清对Prx-I抗体和抗原均呈阳性，但对照血清中没有。数据表明 Prx-I 自身抗体和循环抗原都是用于 NSCLC 血清学诊断的潜在生物标志物。有趣的是，我们发现 Prx-I 由肺腺癌细胞 (A549) 分泌，但不是由非癌肺细胞 (BEAS 2B) 或乳腺癌细胞 (MCF7) 分泌。这项细胞培养研究表明 NSCLC 肿瘤组织分泌 Prx-I 的可能性。 (c) 2005 年欧洲生化学会联合会。由 Elsevier B.V. 出版。保留所有权利。
In eukaryotic cells, peroxiredoxins are both antioxidants and regulators of H2O2-mediated signaling. We previously found that peroxiredoxin-I (Prx-I) was overexpressed in non-small cell lung cancer (NSCLC) tissue. Since overexpressed protein can induce a humoral immune response, we examined whether serum from NSCLC patients exhibited immunoreactivity against Prx-I using Western blotting. We found that 25 (47%) of 53 NSCLC patients tested had autoantibodies against Prx-I in their sera, whereas such activity was detected in 4 (8%) sera from 50 healthy subjects. Prx-I itself was detected in the sera from 18 (34%) of 53 NSCLC patients but in only 1 (2%) serum from 50 controls. Moreover, 17% of NSCLC sera were positive to both Prx-I antibody and antigen but none in control sera. The data indicate both Prx-I autoantibody and circulating antigen are potential biomarkers for use in serological diagnosis of NSCLC. Interestingly enough, we found that Prx-I was secreted by lung adenocarcinoma cells (A549) but not by non-cancer lung cells (BEAS 2B) or breast cancer cells (MCF7). This cell culture study suggests the possibility of Prx-I secretion from NSCLC tumor tissues. (c) 2005 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.