POSITIVE AND NEGATIVE THYMOCYTE SELECTION INDUCED BY DIFFERENT CONCENTRATIONS OF A SINGLE PEPTIDE
POSITIVE AND NEGATIVE THYMOCYTE SELECTION INDUCED BY DIFFERENT CONCENTRATIONS OF A SINGLE PEPTIDE
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DOI：
10.1126/science.8128249
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发表时间：
1994-03-18
期刊：
影响因子：
56.9
通讯作者：
OHASHI, PS
中科院分区：
文献类型：
--
作者：
SEBZDA, E;WALLACE, VA;OHASHI, PS
T淋巴细胞的成熟依赖于胸腺细胞上表达的T细胞受体(TCR)与胸腺内主要组织相容性复合体(MHC)-多肽配体之间的相互作用。导致阴性或阳性选择的多肽-MHC复合体之间的关系尚未确定。在这里，研究了在胎儿胸腺器官培养中，表达针对病毒多肽的特定转基因TCR的胸腺细胞成熟的要求。这种转基因TCR识别的病毒多肽抗原的低浓度可以介导正选择，而高浓度则导致胸腺细胞耐受。这些发现支持胸腺细胞选择的亲和力模型。
T lymphocyte maturation is dependent on interactions between the T cell receptor (TCR) expressed on the developing thymocyte and intrathymic major histocompatibility complex (MHC)-peptide ligands. The relation between the peptide-MHC complex that results in negative or positive selection has not been identified. Here, the requirements for the maturation of thymocytes expressing a defined transgenic TCR specific for a viral peptide are studied in fetal thymic organ culture. Low concentrations of the viral peptide antigen recognized by this transgenic TCR can mediate positive selection, whereas high concentrations result in thymocyte tolerance. These findings support the affinity-avidity model of thymocyte selection.