Construction of a large-insert yeast artificial chromosome library of the rat genome.
Construction of a large-insert yeast artificial chromosome library of the rat genome.
复制标题
大鼠基因组大插入酵母人工染色体文库的构建。
DOI：
10.1007/s003359900412
复制
发表时间：
1997
期刊：
影响因子：
--
通讯作者：
Lander,ES
中科院分区：
文献类型：
--
作者：
Haldi,ML;Lim,P;Kaphingst,K;Akella,U;Whang,J;Lander,ES
The rat is the animal model of choice for the study of many aspects of mammalian physiology. However, the rat has lagged behind the mouse in terms of genetic studies, in large part owing to the lack of appropriate genetic and genomic mapping resources. As part of our ongoing effort to provide mapping resources to the mammalian genetics community, we have constructed the first yeast artificial chromosome (YAC) library of the rat genome. This library consists of approximately 41,500 clones with an average insert size of 820 kb and provides roughly 11-fold coverage. The methods used for preparation of the library are described by Haldi and colleagues (1996). Genomic DNA was prepared from kidney nuclei of Fischer 344 female rats. This DNA was ligated to pRML1 and pRML2 vector arms (Spencer et al. 1993). The ligation product was transformed into the yeast host strain J57D
