Functional genome annotation of Drosophila seminal fluid proteins using transcriptional genetic networks.
Functional genome annotation of Drosophila seminal fluid proteins using transcriptional genetic networks.
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DOI：
10.1017/s0016672311000346
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发表时间：
2011-12
影响因子：
1.5
通讯作者：
Mackay, Trudy F. C.
中科院分区：
文献类型：
--
作者：
Ayroles, Julien F.;Laflamme, Brooke A.;Stone, Eric A.;Wolfner, Mariana F.;Mackay, Trudy F. C.
预测功能基因注释仍然是一个重大的挑战，即使在注释良好的基因组，如酵母和果蝇。一个有前途的，高通量的基因注释方法是使用相关的基因表达模式来注释靶基因的基础上已知的功能的焦点基因。黑腹果蝇转录组在野生近交系中存在遗传差异，转录本之间具有很强的遗传相关性。在这里，我们利用已知的精液蛋白（SFP）基因和其余的遗传变异转录组之间的基因表达的遗传相关性，以确定176个新的候选SFP（cSFP）。我们独立验证了7个cSFP和一个已知SFP基因之间的基因表达相关性，以及在男性生殖组织中的表达。我们认为，这种方法可以扩展到其他系统的基因表达的遗传变异的信息。
Predicting functional gene annotations remains a significant challenge, even in well-annotated genomes such as yeast and Drosophila. One promising, high-throughput method for gene annotation is to use correlated gene expression patterns to annotate target genes based on the known function of focal genes. The Drosophila melanogaster transcriptome varies genetically among wild derived inbred lines, with strong genetic correlations among the transcripts. Here, we leveraged the genetic correlations in gene expression among known seminal fluid protein (SFP) genes and the rest of the genetically varying transcriptome to identify 176 novel candidate SFPs (cSFPs). We independently validated the correlation in gene expression between seven of the cSFPs and a known SFP gene, as well as expression in male reproductive tissues. We argue that this method can be extended to other systems for which information on genetic variation in gene expression is available.