PH-DEPENDENT LYSIS OF LIPOSOMES BY ADENOVIRUS
PH-DEPENDENT LYSIS OF LIPOSOMES BY ADENOVIRUS
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DOI：
10.1021/bi00356a057
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发表时间：
1986-04-22
期刊：
影响因子：
2.9
通讯作者：
PASTAN, I
中科院分区：
文献类型：
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作者：
BLUMENTHAL, R;SETH, P;PASTAN, I
纯化的腺病毒诱导剂量依赖性释放的水溶性标记钙黄绿素和羧基荧光素从脂质体。标记物的释放强烈依赖于pH，并且在低于5 ℃的温度下。在pH = 6左右时释放速率最快。这种pH依赖性与腺病毒对内吞囊泡的破坏和腺病毒在细胞表面诱导的透化作用平行。似乎没有对脂质体的脂质组成的显著依赖性。使用负染色的电子显微镜显示脂质体与腺病毒结合。在某些情况下，脂质体仍然完整，但许多附着在病毒顶点的脂质体似乎溶解了。这些数据支持这样的观点：通过受体介导的内吞作用进入宿主细胞的腺病毒，随后通过pH依赖性破坏内吞囊泡的膜而进入细胞质。
Purified adenovirus induced a dose-dependent release of the water-soluble markers calcein and carboxyfluorescein from liposomes. Marker release was strongly dependent on pH, and at temperatures below 5.degree. C, the rate of release showed an optimum at a pH of about 6. This pH dependence parallels disruption of endocytic vesicles by adenovirus and the permeabilization that adenovirus induces on the cell surface. There did not seem to be a striking dependence on the lipid composition of the liposomes. Electron microscopy using a negative stain shows liposomes bound to adenovirus. In some cases, the liposomes were still intact, but many liposomes, which were attached to the vertices of the virus, appeared lysed. These data support the notion that adenovirus, which enters the host cell by receptor-mediated endocytosis, gains access to the cytoplasm by a subsequent pH-dependent disruption of the membrane of the endocytic vesicle.